Protocol for gene knockdown using siRNA in primary cultured neonatal murine microglia

Protocol for gene knockdown using siRNA in primary cultured neonatal murine microglia

Summary: In this protocol, we describe the small interfering RNA (siRNA)-mediated gene knockdown in primary mouse microglia, providing an approach to investigate functions such as phagocytosis and chemotaxis. The approach includes siRNA design, establishment of mixed glial cultures, microglia isolat...

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Bibliographic Details
Main Authors: Yuma Kato, Sho Takatori, Aika Akahori, Hayato Etani, Yung Ning Chu, Taisuke Tomita
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell isolation
Immunology
Molecular Biology
Gene Expression
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724000327

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http://www.sciencedirect.com/science/article/pii/S2666166724000327

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