A SEVA-based, CRISPR-Cas3-assisted genome engineering approach for Pseudomonas with efficient vector curing
ABSTRACT The development of CRISPR-Cas-based engineering technologies has revolutionized the microbial biotechnology field. Over the years, the Class II Type II CRISPR-Cas9 system has become the gold standard for genome editing in many bacterial hosts. However, the Cas9 system does not allow efficie...
| Principais autores: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | English |
| Publicado em: |
American Society for Microbiology
2023-12-01
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| coleção: | Microbiology Spectrum |
| Assuntos: | |
| Acesso em linha: | https://journals.asm.org/doi/10.1128/spectrum.02707-23 |