Protocol for detecting histidine polyphosphate modification of human proteins via MBP-tagged expression in E. coli

Protocol for detecting histidine polyphosphate modification of human proteins via MBP-tagged expression in E. coli

Summary: Polyphosphate exhibits a unique post-translational modification-like function, known as histidine polyphosphate modification (HPM), marked by a robust non-covalent interaction with histidine repeat proteins. Here, we present a protocol for detecting HPM of human proteins via maltose-binding...

Full description

Bibliographic Details
Main Authors: Kirsten Lehotsky, Nolan Neville, Zongchao Jia
Format: Article
Language:English
Published: Elsevier 2024-06-01
Series:STAR Protocols
Subjects:
Molecular Biology
Protein Biochemistry
Protein expression and purification
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724001126

Internet

http://www.sciencedirect.com/science/article/pii/S2666166724001126

Similar Items