Determination of cold-adapted influenza virus (Orthomyxoviridae: <i>Alphainfluenzavirus</i>) polymerase activity by the minigenome method with a fluorescent protein

Determination of cold-adapted influenza virus (Orthomyxoviridae: <i>Alphainfluenzavirus</i>) polymerase activity by the minigenome method with a fluorescent protein

Introduction. Polymerase proteins PB1 and PB2 determine the cold-adapted phenotype of the influenza virus A/Krasnodar/101/35/59 (H2N2), as was shown earlier. Objective. The development of the reporter construct to determine the activity of viral polymerase at 33 and 37 °C using the minigenome met...

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Bibliographic Details
Main Authors: Pavel A. Ivanov, Aleksandr V. Lyashko, Vladimir Y. Kost, Natalia F. Lomakina, Artyom A. Rtishchev, Nataliya I. Bunkova, Tatiana A. Timofeeva, Marina A. Balanova, Stepan A. Ionov, Dmitry V. Gorikov, Stanislav G. Markushin
Format: Article
Language:English
Published: Central Research Institute for Epidemiology 2023-12-01
Series:Вопросы вирусологии
Subjects:
reverse genetics
influenza a virus
influenza virus polymerase
influenza virus minigenome
green fluorescent protein
cold-adapted influenza virus
Online Access:https://virusjour.crie.ru/jour/article/viewFile/16588/849

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https://virusjour.crie.ru/jour/article/viewFile/16588/849

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