Usnic Acid Potassium Salt: Evaluation of the Acute Toxicity and Antinociceptive Effect in Murine Model
To obtain usnic acid potassium salt (PS-UA), the usnic acid (UA) was extracted and purified from the lichen <i>Cladonia substellata</i>, and modified to produce PS-UA. The structure was determined by <sup>1</sup>H-NMR, IR and elemental analysis, ratified through computational...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/11/2042 |
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| author | Hallysson Douglas A. Araújo José G. Silva Júnior João R. Saturnino Oliveira Maria Helena M. L. Ribeiro Mônica C. Barroso Martins Marcos A. Cavalcanti Bezerra André Lima Aires Mônica C. P. Azevedo Albuquerque Mário R. Melo-Júnior Nicodemos T. Pontes Filho Eugênia C. Pereira Diego J. Raposo Silva Janaína V. dos Anjos Emerson Peter S. Falcão Nicácio H. Silva Vera L. Menezes Lima |
| author_facet | Hallysson Douglas A. Araújo José G. Silva Júnior João R. Saturnino Oliveira Maria Helena M. L. Ribeiro Mônica C. Barroso Martins Marcos A. Cavalcanti Bezerra André Lima Aires Mônica C. P. Azevedo Albuquerque Mário R. Melo-Júnior Nicodemos T. Pontes Filho Eugênia C. Pereira Diego J. Raposo Silva Janaína V. dos Anjos Emerson Peter S. Falcão Nicácio H. Silva Vera L. Menezes Lima |
| author_sort | Hallysson Douglas A. Araújo |
| collection | DOAJ |
| description | To obtain usnic acid potassium salt (PS-UA), the usnic acid (UA) was extracted and purified from the lichen <i>Cladonia substellata</i>, and modified to produce PS-UA. The structure was determined by <sup>1</sup>H-NMR, IR and elemental analysis, ratified through computational models, as well as identification the site of K<sup>+</sup> insertion in the molecule. Antinociceptive activity was detected through contortions in mice induced by acetic acid and formalin (phases I and II) after treatments with 10 and 20 mg/kg of PS-UA, indicating interference in both non-inflammatory and inflammatory pain. After oral administration at doses of 500, 1000 and 2000 mg/kg, no deaths of mice with treatments below 2000 mg/kg were observed. Except for body weight gain, food and water consumption decreased with treatments of 1000 and 2000 mg/kg, and the number of segmented leukocytes was higher for both treatments. Regarding serum levels, cholesterol and triglycerides decreased, however, there was an increase in hepatic transaminases with both treatments. Liver and kidney histological changes were detected in treatments of 2000 mg/kg, while the spleen was preserved. The PS-UA demonstrated antinociceptive activity while the acute toxicity at the concentration of 2000 mg/kg was the only dose that presented morphological changes in the liver and kidney. |
| first_indexed | 2024-04-13T02:50:55Z |
| format | Article |
| id | doaj.art-e22b4472a254440999f9ab6659194d93 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-13T02:50:55Z |
| publishDate | 2019-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-e22b4472a254440999f9ab6659194d932022-12-22T03:05:51ZengMDPI AGMolecules1420-30492019-05-012411204210.3390/molecules24112042molecules24112042Usnic Acid Potassium Salt: Evaluation of the Acute Toxicity and Antinociceptive Effect in Murine ModelHallysson Douglas A. Araújo0José G. Silva Júnior1João R. Saturnino Oliveira2Maria Helena M. L. Ribeiro3Mônica C. Barroso Martins4Marcos A. Cavalcanti Bezerra5André Lima Aires6Mônica C. P. Azevedo Albuquerque7Mário R. Melo-Júnior8Nicodemos T. Pontes Filho9Eugênia C. Pereira10Diego J. Raposo Silva11Janaína V. dos Anjos12Emerson Peter S. Falcão13Nicácio H. Silva14Vera L. Menezes Lima15Centro de Biociências–Departamento de Bioquímica, Universidade Federal de Pernambuco, Avenida Prof. Moraes Rego, 1235, Cidade Universitária, Recife, PE 50.670-501, BrazilCentro de Biociências–Departamento de Bioquímica, Universidade Federal de Pernambuco, Avenida Prof. Moraes Rego, 1235, Cidade Universitária, Recife, PE 50.670-501, BrazilCentro de Biociências–Departamento de Bioquímica, Universidade Federal de Pernambuco, Avenida Prof. Moraes Rego, 1235, Cidade Universitária, Recife, PE 50.670-501, BrazilLaboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco. Avenida Prof. Moraes Rego, 1235 Cidade Universitária, Recife, PE 50.670-501, BrazilCentro de Biociências–Departamento de Bioquímica, Universidade Federal de Pernambuco, Avenida Prof. Moraes Rego, 1235, Cidade Universitária, Recife, PE 50.670-501, BrazilCentro de Biociências–Departamento de Biofísica e Radiobiologia, Universidade Federal de Pernambuco. Avenida Prof. Moraes Rego, 1235 Cidade Universitária, Recife, PE 50.670-501, BrazilLaboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco. Avenida Prof. Moraes Rego, 1235 Cidade Universitária, Recife, PE 50.670-501, BrazilLaboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco. Avenida Prof. Moraes Rego, 1235 Cidade Universitária, Recife, PE 50.670-501, BrazilLaboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco. Avenida Prof. Moraes Rego, 1235 Cidade Universitária, Recife, PE 50.670-501, BrazilLaboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco. Avenida Prof. Moraes Rego, 1235 Cidade Universitária, Recife, PE 50.670-501, BrazilDepartamento de Ciências Geográficas, Centro de Filosofia e Ciências Humanas, Universidade Federal de Pernambuco, Recife, PE 50.740-530, BrazilDepartamento de Química Fundamental, Universidade Federal de Pernambuco, Recife, PE 50.740-540, BrazilDepartamento de Química Fundamental, Universidade Federal de Pernambuco, Recife, PE 50.740-540, BrazilLaboratório de Síntese e Isolamento Molecular, Centro Acadêmico de Vitória de Santo Antão, Universidade Federal de Pernambuco, Vitória de Santo Antão, PE 55.608-680, BrazilCentro de Biociências–Departamento de Bioquímica, Universidade Federal de Pernambuco, Avenida Prof. Moraes Rego, 1235, Cidade Universitária, Recife, PE 50.670-501, BrazilCentro de Biociências–Departamento de Bioquímica, Universidade Federal de Pernambuco, Avenida Prof. Moraes Rego, 1235, Cidade Universitária, Recife, PE 50.670-501, BrazilTo obtain usnic acid potassium salt (PS-UA), the usnic acid (UA) was extracted and purified from the lichen <i>Cladonia substellata</i>, and modified to produce PS-UA. The structure was determined by <sup>1</sup>H-NMR, IR and elemental analysis, ratified through computational models, as well as identification the site of K<sup>+</sup> insertion in the molecule. Antinociceptive activity was detected through contortions in mice induced by acetic acid and formalin (phases I and II) after treatments with 10 and 20 mg/kg of PS-UA, indicating interference in both non-inflammatory and inflammatory pain. After oral administration at doses of 500, 1000 and 2000 mg/kg, no deaths of mice with treatments below 2000 mg/kg were observed. Except for body weight gain, food and water consumption decreased with treatments of 1000 and 2000 mg/kg, and the number of segmented leukocytes was higher for both treatments. Regarding serum levels, cholesterol and triglycerides decreased, however, there was an increase in hepatic transaminases with both treatments. Liver and kidney histological changes were detected in treatments of 2000 mg/kg, while the spleen was preserved. The PS-UA demonstrated antinociceptive activity while the acute toxicity at the concentration of 2000 mg/kg was the only dose that presented morphological changes in the liver and kidney.https://www.mdpi.com/1420-3049/24/11/2042lichen<i>Cladonia substellata</i>usnic acid derivativesantinociceptive activity (phase I and II)toxicological surveysoluble drughistopathology |
| spellingShingle | Hallysson Douglas A. Araújo José G. Silva Júnior João R. Saturnino Oliveira Maria Helena M. L. Ribeiro Mônica C. Barroso Martins Marcos A. Cavalcanti Bezerra André Lima Aires Mônica C. P. Azevedo Albuquerque Mário R. Melo-Júnior Nicodemos T. Pontes Filho Eugênia C. Pereira Diego J. Raposo Silva Janaína V. dos Anjos Emerson Peter S. Falcão Nicácio H. Silva Vera L. Menezes Lima Usnic Acid Potassium Salt: Evaluation of the Acute Toxicity and Antinociceptive Effect in Murine Model Molecules lichen <i>Cladonia substellata</i> usnic acid derivatives antinociceptive activity (phase I and II) toxicological survey soluble drug histopathology |
| title | Usnic Acid Potassium Salt: Evaluation of the Acute Toxicity and Antinociceptive Effect in Murine Model |
| title_full | Usnic Acid Potassium Salt: Evaluation of the Acute Toxicity and Antinociceptive Effect in Murine Model |
| title_fullStr | Usnic Acid Potassium Salt: Evaluation of the Acute Toxicity and Antinociceptive Effect in Murine Model |
| title_full_unstemmed | Usnic Acid Potassium Salt: Evaluation of the Acute Toxicity and Antinociceptive Effect in Murine Model |
| title_short | Usnic Acid Potassium Salt: Evaluation of the Acute Toxicity and Antinociceptive Effect in Murine Model |
| title_sort | usnic acid potassium salt evaluation of the acute toxicity and antinociceptive effect in murine model |
| topic | lichen <i>Cladonia substellata</i> usnic acid derivatives antinociceptive activity (phase I and II) toxicological survey soluble drug histopathology |
| url | https://www.mdpi.com/1420-3049/24/11/2042 |
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