Differential Effects of Alarmins on Human and Mouse Basophils
Epithelial-derived alarmins (IL-33, TSLP, and IL-25) play an upstream role in the pathogenesis of asthma. Basophil-derived cytokines are a pivotal component of allergic inflammation. We evaluated the in vitro effects of IL-33, TSLP, and IL-25, alone and in combination with IL-3 on purified periphera...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.894163/full |
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| author | Adriana R. Gambardella Adriana R. Gambardella Remo Poto Remo Poto Remo Poto Remo Poto Valentina Tirelli John T. Schroeder Gianni Marone Gianni Marone Gianni Marone Gianni Marone Fabrizio Mattei Gilda Varricchi Gilda Varricchi Gilda Varricchi Gilda Varricchi Giovanna Schiavoni |
| author_facet | Adriana R. Gambardella Adriana R. Gambardella Remo Poto Remo Poto Remo Poto Remo Poto Valentina Tirelli John T. Schroeder Gianni Marone Gianni Marone Gianni Marone Gianni Marone Fabrizio Mattei Gilda Varricchi Gilda Varricchi Gilda Varricchi Gilda Varricchi Giovanna Schiavoni |
| author_sort | Adriana R. Gambardella |
| collection | DOAJ |
| description | Epithelial-derived alarmins (IL-33, TSLP, and IL-25) play an upstream role in the pathogenesis of asthma. Basophil-derived cytokines are a pivotal component of allergic inflammation. We evaluated the in vitro effects of IL-33, TSLP, and IL-25, alone and in combination with IL-3 on purified peripheral blood human basophils (hBaso) and bone marrow-derived mouse basophils (mBaso) in modulating the production of IL-4, IL-13, CXCL8 or the mouse CXCL8 equivalents CXCL1 and CXCL2. IL-3 and IL-33, but not TSLP and IL-25, concentration-dependently induced IL-4, IL-13, and CXCL8 release from hBaso. IL-3 synergistically potentiated the release of cytokines induced by IL-33 from hBaso. In mBaso, IL-3 and IL-33 rapidly induced IL-4 and IL-13 mRNA expression and protein release. IL-33, but not IL-3, induced CXCL2 and CXCL1 from mBaso. Differently from hBaso, TSLP induced IL-4, IL-13, CXCL1 and CXCL2 mRNA expression and protein release from mBaso. IL-25 had no effect on IL-4, IL-13, and CXCL1/CXCL2 mRNA expression and protein release even in the presence of IL-3. No synergism was observed between IL-3 and either IL-25 or TSLP. IL-3 inhibited both TSLP- and IL-33-induced CXCL1 and CXCL2 release from mBaso. Our results highlight some similarities and marked differences between the effects of IL-3 and alarmins on the release of cytokines from human and mouse basophils. |
| first_indexed | 2024-04-13T17:26:26Z |
| format | Article |
| id | doaj.art-e251f6f1eaba4ebca712534413a67c34 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-13T17:26:26Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-e251f6f1eaba4ebca712534413a67c342022-12-22T02:37:47ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-05-011310.3389/fimmu.2022.894163894163Differential Effects of Alarmins on Human and Mouse BasophilsAdriana R. Gambardella0Adriana R. Gambardella1Remo Poto2Remo Poto3Remo Poto4Remo Poto5Valentina Tirelli6John T. Schroeder7Gianni Marone8Gianni Marone9Gianni Marone10Gianni Marone11Fabrizio Mattei12Gilda Varricchi13Gilda Varricchi14Gilda Varricchi15Gilda Varricchi16Giovanna Schiavoni17Department of Oncology and Molecular Medicine, Istituto Superiore di Sanità (ISS), Rome, ItalyDepartment of Translational Medical Sciences, University of Naples Federico II, Naples, ItalyDepartment of Oncology and Molecular Medicine, Istituto Superiore di Sanità (ISS), Rome, ItalyDepartment of Translational Medical Sciences, University of Naples Federico II, Naples, ItalyCenter for Basic and Clinical Immunology Research (CISI), University of Naples Federico II, Naples, ItalyWorld Allergy Organization (WAO) Center of Excellence, Naples, ItalyCore Facilities, Istituto Superiore di Sanità (ISS), Rome, ItalyDivision of Allergy and Clinical Immunology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, United StatesDepartment of Translational Medical Sciences, University of Naples Federico II, Naples, ItalyCenter for Basic and Clinical Immunology Research (CISI), University of Naples Federico II, Naples, ItalyWorld Allergy Organization (WAO) Center of Excellence, Naples, ItalyInstitute of Experimental Endocrinology and Oncology (IEOS), National Research Council, Naples, ItalyDepartment of Oncology and Molecular Medicine, Istituto Superiore di Sanità (ISS), Rome, ItalyDepartment of Translational Medical Sciences, University of Naples Federico II, Naples, ItalyCenter for Basic and Clinical Immunology Research (CISI), University of Naples Federico II, Naples, ItalyWorld Allergy Organization (WAO) Center of Excellence, Naples, ItalyInstitute of Experimental Endocrinology and Oncology (IEOS), National Research Council, Naples, ItalyDepartment of Oncology and Molecular Medicine, Istituto Superiore di Sanità (ISS), Rome, ItalyEpithelial-derived alarmins (IL-33, TSLP, and IL-25) play an upstream role in the pathogenesis of asthma. Basophil-derived cytokines are a pivotal component of allergic inflammation. We evaluated the in vitro effects of IL-33, TSLP, and IL-25, alone and in combination with IL-3 on purified peripheral blood human basophils (hBaso) and bone marrow-derived mouse basophils (mBaso) in modulating the production of IL-4, IL-13, CXCL8 or the mouse CXCL8 equivalents CXCL1 and CXCL2. IL-3 and IL-33, but not TSLP and IL-25, concentration-dependently induced IL-4, IL-13, and CXCL8 release from hBaso. IL-3 synergistically potentiated the release of cytokines induced by IL-33 from hBaso. In mBaso, IL-3 and IL-33 rapidly induced IL-4 and IL-13 mRNA expression and protein release. IL-33, but not IL-3, induced CXCL2 and CXCL1 from mBaso. Differently from hBaso, TSLP induced IL-4, IL-13, CXCL1 and CXCL2 mRNA expression and protein release from mBaso. IL-25 had no effect on IL-4, IL-13, and CXCL1/CXCL2 mRNA expression and protein release even in the presence of IL-3. No synergism was observed between IL-3 and either IL-25 or TSLP. IL-3 inhibited both TSLP- and IL-33-induced CXCL1 and CXCL2 release from mBaso. Our results highlight some similarities and marked differences between the effects of IL-3 and alarmins on the release of cytokines from human and mouse basophils.https://www.frontiersin.org/articles/10.3389/fimmu.2022.894163/fullallergyasthmabasophilsIL-4IL-13IL-25 |
| spellingShingle | Adriana R. Gambardella Adriana R. Gambardella Remo Poto Remo Poto Remo Poto Remo Poto Valentina Tirelli John T. Schroeder Gianni Marone Gianni Marone Gianni Marone Gianni Marone Fabrizio Mattei Gilda Varricchi Gilda Varricchi Gilda Varricchi Gilda Varricchi Giovanna Schiavoni Differential Effects of Alarmins on Human and Mouse Basophils Frontiers in Immunology allergy asthma basophils IL-4 IL-13 IL-25 |
| title | Differential Effects of Alarmins on Human and Mouse Basophils |
| title_full | Differential Effects of Alarmins on Human and Mouse Basophils |
| title_fullStr | Differential Effects of Alarmins on Human and Mouse Basophils |
| title_full_unstemmed | Differential Effects of Alarmins on Human and Mouse Basophils |
| title_short | Differential Effects of Alarmins on Human and Mouse Basophils |
| title_sort | differential effects of alarmins on human and mouse basophils |
| topic | allergy asthma basophils IL-4 IL-13 IL-25 |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.894163/full |
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