Neurotoxic non-protein amino acids in commercially harvested Lobsters (Homarus americanus H. Milne-Edwards)
Abstract Cyanobacteria produce neurotoxic non-protein amino acids (NPAAs) that accumulate in ecosystems and food webs. American lobsters (Homarus americanus H. Milne-Edwards) are one of the most valuable seafood industries in Canada with exports valued at > $2 billion. Two previous studies have a...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-04-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-58778-1 |
| _version_ | 1797219899879194624 |
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| author | Pawanjit K. Sandhu Julia T. Solonenka Susan J. Murch |
| author_facet | Pawanjit K. Sandhu Julia T. Solonenka Susan J. Murch |
| author_sort | Pawanjit K. Sandhu |
| collection | DOAJ |
| description | Abstract Cyanobacteria produce neurotoxic non-protein amino acids (NPAAs) that accumulate in ecosystems and food webs. American lobsters (Homarus americanus H. Milne-Edwards) are one of the most valuable seafood industries in Canada with exports valued at > $2 billion. Two previous studies have assessed the occurrence of β-N-methylamino-L-alanine (BMAA) in a small number of lobster tissues but a complete study has not previously been undertaken. We measured NPAAs in eyeballs, brain, legs, claws, tails, and eggs of 4 lobsters per year for the 2021 and 2022 harvests. Our study included 4 male and 4 female lobsters. We detected BMAA and its isomers, N-(2-aminoethyl)glycine (AEG), 2,4-diaminobutyric acid (DAB) and β-aminomethyl-L-alanine (BAMA) by a fully validated reverse phase chromatography—tandem mass spectrometry method. We quantified BMAA, DAB, AEG and BAMA in all of the lobster tissues. Our quantification data varied by individual lobster, sex and collection year. Significantly more BMAA was quantified in lobsters harvested in 2021 than 2022. Interestingly, more BAMA was quantified in lobsters harvested in 2022 than 2021. Monitoring of lobster harvests for cyanobacterial neurotoxins when harmful algal bloom events occur could mitigate risks to human health. |
| first_indexed | 2024-04-24T12:40:59Z |
| format | Article |
| id | doaj.art-e266bb086448403bb83ae53e415720c2 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T12:40:59Z |
| publishDate | 2024-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-e266bb086448403bb83ae53e415720c22024-04-07T11:17:17ZengNature PortfolioScientific Reports2045-23222024-04-011411910.1038/s41598-024-58778-1Neurotoxic non-protein amino acids in commercially harvested Lobsters (Homarus americanus H. Milne-Edwards)Pawanjit K. Sandhu0Julia T. Solonenka1Susan J. Murch2Department of Chemistry, University of British Columbia, Syilx Okanagan Nation TerritoryDepartment of Chemistry, University of British Columbia, Syilx Okanagan Nation TerritoryDepartment of Chemistry, University of British Columbia, Syilx Okanagan Nation TerritoryAbstract Cyanobacteria produce neurotoxic non-protein amino acids (NPAAs) that accumulate in ecosystems and food webs. American lobsters (Homarus americanus H. Milne-Edwards) are one of the most valuable seafood industries in Canada with exports valued at > $2 billion. Two previous studies have assessed the occurrence of β-N-methylamino-L-alanine (BMAA) in a small number of lobster tissues but a complete study has not previously been undertaken. We measured NPAAs in eyeballs, brain, legs, claws, tails, and eggs of 4 lobsters per year for the 2021 and 2022 harvests. Our study included 4 male and 4 female lobsters. We detected BMAA and its isomers, N-(2-aminoethyl)glycine (AEG), 2,4-diaminobutyric acid (DAB) and β-aminomethyl-L-alanine (BAMA) by a fully validated reverse phase chromatography—tandem mass spectrometry method. We quantified BMAA, DAB, AEG and BAMA in all of the lobster tissues. Our quantification data varied by individual lobster, sex and collection year. Significantly more BMAA was quantified in lobsters harvested in 2021 than 2022. Interestingly, more BAMA was quantified in lobsters harvested in 2022 than 2021. Monitoring of lobster harvests for cyanobacterial neurotoxins when harmful algal bloom events occur could mitigate risks to human health.https://doi.org/10.1038/s41598-024-58778-1Lobsterβ-methylamino-alanineNon-protein amino acidNeurotoxinNew Brunswick |
| spellingShingle | Pawanjit K. Sandhu Julia T. Solonenka Susan J. Murch Neurotoxic non-protein amino acids in commercially harvested Lobsters (Homarus americanus H. Milne-Edwards) Scientific Reports Lobster β-methylamino-alanine Non-protein amino acid Neurotoxin New Brunswick |
| title | Neurotoxic non-protein amino acids in commercially harvested Lobsters (Homarus americanus H. Milne-Edwards) |
| title_full | Neurotoxic non-protein amino acids in commercially harvested Lobsters (Homarus americanus H. Milne-Edwards) |
| title_fullStr | Neurotoxic non-protein amino acids in commercially harvested Lobsters (Homarus americanus H. Milne-Edwards) |
| title_full_unstemmed | Neurotoxic non-protein amino acids in commercially harvested Lobsters (Homarus americanus H. Milne-Edwards) |
| title_short | Neurotoxic non-protein amino acids in commercially harvested Lobsters (Homarus americanus H. Milne-Edwards) |
| title_sort | neurotoxic non protein amino acids in commercially harvested lobsters homarus americanus h milne edwards |
| topic | Lobster β-methylamino-alanine Non-protein amino acid Neurotoxin New Brunswick |
| url | https://doi.org/10.1038/s41598-024-58778-1 |
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