Evaluation of therapeutic use of a combination of pentoxifylline and vitamin E in radiation-induced renal fibrosis
Abstract Radiation-induced renal fibrosis (RIRF) is a progressive, irreversible condition causing chronic kidney disease. Pentoxifylline (PTX) and vitamin E may mitigate radiation-induced damage and fibrosis. This study assesses their effectiveness. We used four groups, each with six rats: radiation...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-57850-0 |
| _version_ | 1827310303395708928 |
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| author | Volkan Demircan Caglar Guzel Gulistan Sanem Sarıbas Serap Catlı Dinc Serhat Cetin Ozlem Gulbahar Petek Erpolat Cigdem Elmas Huseyin Bora |
| author_facet | Volkan Demircan Caglar Guzel Gulistan Sanem Sarıbas Serap Catlı Dinc Serhat Cetin Ozlem Gulbahar Petek Erpolat Cigdem Elmas Huseyin Bora |
| author_sort | Volkan Demircan |
| collection | DOAJ |
| description | Abstract Radiation-induced renal fibrosis (RIRF) is a progressive, irreversible condition causing chronic kidney disease. Pentoxifylline (PTX) and vitamin E may mitigate radiation-induced damage and fibrosis. This study assesses their effectiveness. We used four groups, each with six rats: radiation therapy alone (RT-only), radiation therapy plus drug treatment (RT + drug), drug treatment alone (drug-only), and a control group. Rats were monitored for three months, with weight measurements every four weeks. Afterward, rats were analyzed biochemically and histologically, with blood and tissue samples taken for statistical comparison. No significant differences in serum creatinine levels and body weight were observed. RT-only group had more severe kidney tubule effects. Histomorphological, immunohistochemical, and TUNEL analyses showed significant RIRF mitigation in the RT + drug group. Our study highlighted molecular pathways (SMAD, TGF-beta, VEGF) and histological markers (collagens, a-SMA, fibronectin, metalloproteinases) associated with RIRF. PTX and vitamin E reduced ionizing radiation's impact on renal cells and mitigated radiation-induced kidney fibrosis. Further human studies are needed to confirm these findings. |
| first_indexed | 2024-04-24T19:58:39Z |
| format | Article |
| id | doaj.art-e26c2c76647e4dcebb1907f1a5e5d5be |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T19:58:39Z |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-e26c2c76647e4dcebb1907f1a5e5d5be2024-03-24T12:15:25ZengNature PortfolioScientific Reports2045-23222024-03-0114111410.1038/s41598-024-57850-0Evaluation of therapeutic use of a combination of pentoxifylline and vitamin E in radiation-induced renal fibrosisVolkan Demircan0Caglar Guzel1Gulistan Sanem Sarıbas2Serap Catlı Dinc3Serhat Cetin4Ozlem Gulbahar5Petek Erpolat6Cigdem Elmas7Huseyin Bora8Radiation Oncology Department, Acibadem Altunizade/Atasehir HospitalRadiation Oncology Department, Gazi University Medical FacultyHistology and Embryology Department, Health Sciences University Gulhane Medical FacultyRadiation Oncology Department, Gazi University Medical FacultyUrology Department, Gazi University Medical FacultyBiochemistry Department, Gazi University Medical FacultyRadiation Oncology Department, Gazi University Medical FacultyHistology Department, Gazi University Medical FacultyRadiation Oncology Department, Gazi University Medical FacultyAbstract Radiation-induced renal fibrosis (RIRF) is a progressive, irreversible condition causing chronic kidney disease. Pentoxifylline (PTX) and vitamin E may mitigate radiation-induced damage and fibrosis. This study assesses their effectiveness. We used four groups, each with six rats: radiation therapy alone (RT-only), radiation therapy plus drug treatment (RT + drug), drug treatment alone (drug-only), and a control group. Rats were monitored for three months, with weight measurements every four weeks. Afterward, rats were analyzed biochemically and histologically, with blood and tissue samples taken for statistical comparison. No significant differences in serum creatinine levels and body weight were observed. RT-only group had more severe kidney tubule effects. Histomorphological, immunohistochemical, and TUNEL analyses showed significant RIRF mitigation in the RT + drug group. Our study highlighted molecular pathways (SMAD, TGF-beta, VEGF) and histological markers (collagens, a-SMA, fibronectin, metalloproteinases) associated with RIRF. PTX and vitamin E reduced ionizing radiation's impact on renal cells and mitigated radiation-induced kidney fibrosis. Further human studies are needed to confirm these findings.https://doi.org/10.1038/s41598-024-57850-0 |
| spellingShingle | Volkan Demircan Caglar Guzel Gulistan Sanem Sarıbas Serap Catlı Dinc Serhat Cetin Ozlem Gulbahar Petek Erpolat Cigdem Elmas Huseyin Bora Evaluation of therapeutic use of a combination of pentoxifylline and vitamin E in radiation-induced renal fibrosis Scientific Reports |
| title | Evaluation of therapeutic use of a combination of pentoxifylline and vitamin E in radiation-induced renal fibrosis |
| title_full | Evaluation of therapeutic use of a combination of pentoxifylline and vitamin E in radiation-induced renal fibrosis |
| title_fullStr | Evaluation of therapeutic use of a combination of pentoxifylline and vitamin E in radiation-induced renal fibrosis |
| title_full_unstemmed | Evaluation of therapeutic use of a combination of pentoxifylline and vitamin E in radiation-induced renal fibrosis |
| title_short | Evaluation of therapeutic use of a combination of pentoxifylline and vitamin E in radiation-induced renal fibrosis |
| title_sort | evaluation of therapeutic use of a combination of pentoxifylline and vitamin e in radiation induced renal fibrosis |
| url | https://doi.org/10.1038/s41598-024-57850-0 |
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