A protocol for quantifying lipid peroxidation in cellular systems by F2-isoprostane analysis.

Cellular systems are essential model systems to study reactive oxygen species and oxidative damage but there are widely accepted technical difficulties with available methods for quantifying endogenous oxidative damage in these systems. Here we present a stable isotope dilution UPLC-MS/MS protocol f...

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Bibliographic Details
Main Authors: Christiaan F Labuschagne, Niels J F van den Broek, Pjotr Postma, Ruud Berger, Arjan B Brenkman
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3828286?pdf=render