Stereochemical course of hydrolytic reaction catalyzed by alpha-galactosidase from cold adaptable marine bacterium of genus Pseudoalteromonas

Stereochemical course of hydrolytic reaction catalyzed by alpha-galactosidase from cold adaptable marine bacterium of genus Pseudoalteromonas

The recombinant α-galactosidase of the marine bacterium (α-PsGal) was synthesized with the use of the plasmid 40Gal, consisting of plasmid pET-40b (+) (Novagen) and the gene corresponding to the open reading frame of the mature α-galactosidase of marine bacterium Pseudoalteromonas sp. KMM 701, trans...

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Bibliographic Details
Main Authors: Irina Yu Bakunina, Larissa A Balabanova, Vasiliy A Golotin, Lyubov V Slepchenko, Vladimir V Isakov, Valeriy A Rasskazov
Format: Article
Language:English
Published: Frontiers Media S.A. 2014-10-01
Series:Frontiers in Chemistry
Subjects:
stereochemistry
α-galactosidase
marine bacterium Pseudoalteromonas sp.
1H NMR spectra
hydrolysis of glycosides
α-anomer
Online Access:http://journal.frontiersin.org/Journal/10.3389/fchem.2014.00089/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fchem.2014.00089/full

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