A novel imaging method revealed phosphatidylinositol 3,5-bisphosphate-rich domains in the endosome/lysosome membrane

A novel imaging method revealed phosphatidylinositol 3,5-bisphosphate-rich domains in the endosome/lysosome membrane

We developed a new method to observe distribution of phosphatidylinositol 3,5-bisphosphate [PtdIns(3,5)P2] using electron microscopy. In freeze-fracture replicas of quick-frozen samples, PtdIns(3,5)P2 was labeled specifically using recombinant ATG18 tagged with glutathione S-transferase and 4×FLAG,...

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Bibliographic Details
Main Authors: Sho Takatori, Toyoshi Fujimoto
Format: Article
Language:English
Published: Taylor & Francis Group 2016-03-01
Series:Communicative & Integrative Biology
Subjects:
electron microscopy
endosome
freeze-fracture
hyperosmotic stress
lysosome
membrane domain
phosphatidylinositol 3,5-bisphosphate
vacuole
Online Access:http://dx.doi.org/10.1080/19420889.2016.1145319

Internet

http://dx.doi.org/10.1080/19420889.2016.1145319

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