Mechanical barriers and transforming growth factor beta inhibitor on epidural fibrosis in a rabbit laminectomy model
Abstract Background TGF-β has been described as a mediator of fibrosis and scarring. Several studies achieved reduction in experimental scarring through the inhibition of TGF-β. Fibroblasts have been defined as the cell population originating fibrosis, blocking fibroblast invasion may impair epidura...
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| Format: | Article |
| Language: | English |
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BMC
2018-04-01
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| Series: | Journal of Orthopaedic Surgery and Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13018-018-0781-6 |
| _version_ | 1811186114843639808 |
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| author | Juan N. Albiñana-Cunningham Purificación Ripalda-Cemboráin Tania Labiano José I. Echeveste Froilán Granero-Moltó Matías Alfonso-Olmos |
| author_facet | Juan N. Albiñana-Cunningham Purificación Ripalda-Cemboráin Tania Labiano José I. Echeveste Froilán Granero-Moltó Matías Alfonso-Olmos |
| author_sort | Juan N. Albiñana-Cunningham |
| collection | DOAJ |
| description | Abstract Background TGF-β has been described as a mediator of fibrosis and scarring. Several studies achieved reduction in experimental scarring through the inhibition of TGF-β. Fibroblasts have been defined as the cell population originating fibrosis, blocking fibroblast invasion may impair epidural fibrosis appearance. For this purpose, biocompatible materials used as mechanical barriers and a TGF-β inhibitor peptide were evaluated in the reduction of epidural fibrosis. Methods A L6 laminectomy was performed in 40 New Zealand white rabbits. Divided into four groups, each rabbit was assigned to receive either collagen sponge scaffold (CS group), gelatin-based gel (GCP group), P144® (iTGFβ group), or left untreated (control group). Four weeks after surgery, cell density, collagen content, and new bone formation of the scar area were determined by histomorphometry. Two experienced pathologists scored dura mater adhesion, scar density, and inflammatory infiltrate in a blinded manner. Results In all groups, laminectomy site was filled with fibrous tissue and the dura mater presented adhesions. Only GCP group presented a significant reduction in collagen content and scar density. Conclusion GCP treatment reduces epidural fibrosis although did not prevent dura mater adhesion completely. |
| first_indexed | 2024-04-11T13:41:20Z |
| format | Article |
| id | doaj.art-e2ec2593af474ac180e4fdcb00030d82 |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-04-11T13:41:20Z |
| publishDate | 2018-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-e2ec2593af474ac180e4fdcb00030d822022-12-22T04:21:16ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2018-04-011311710.1186/s13018-018-0781-6Mechanical barriers and transforming growth factor beta inhibitor on epidural fibrosis in a rabbit laminectomy modelJuan N. Albiñana-Cunningham0Purificación Ripalda-Cemboráin1Tania Labiano2José I. Echeveste3Froilán Granero-Moltó4Matías Alfonso-Olmos5Orthopaedic Surgery and Traumatology Department, Clínica Universidad de NavarraOrthopaedic Surgery and Traumatology Department, Clínica Universidad de NavarraPathology Department, Complejo Hospitalario de NavarraPathology Department, Clínica Universidad de NavarraOrthopaedic Surgery and Traumatology Department, Clínica Universidad de NavarraOrthopaedic Surgery and Traumatology Department, Clínica Universidad de NavarraAbstract Background TGF-β has been described as a mediator of fibrosis and scarring. Several studies achieved reduction in experimental scarring through the inhibition of TGF-β. Fibroblasts have been defined as the cell population originating fibrosis, blocking fibroblast invasion may impair epidural fibrosis appearance. For this purpose, biocompatible materials used as mechanical barriers and a TGF-β inhibitor peptide were evaluated in the reduction of epidural fibrosis. Methods A L6 laminectomy was performed in 40 New Zealand white rabbits. Divided into four groups, each rabbit was assigned to receive either collagen sponge scaffold (CS group), gelatin-based gel (GCP group), P144® (iTGFβ group), or left untreated (control group). Four weeks after surgery, cell density, collagen content, and new bone formation of the scar area were determined by histomorphometry. Two experienced pathologists scored dura mater adhesion, scar density, and inflammatory infiltrate in a blinded manner. Results In all groups, laminectomy site was filled with fibrous tissue and the dura mater presented adhesions. Only GCP group presented a significant reduction in collagen content and scar density. Conclusion GCP treatment reduces epidural fibrosis although did not prevent dura mater adhesion completely.http://link.springer.com/article/10.1186/s13018-018-0781-6Epidural fibrosisLaminectomyTGF-βBiomaterials |
| spellingShingle | Juan N. Albiñana-Cunningham Purificación Ripalda-Cemboráin Tania Labiano José I. Echeveste Froilán Granero-Moltó Matías Alfonso-Olmos Mechanical barriers and transforming growth factor beta inhibitor on epidural fibrosis in a rabbit laminectomy model Journal of Orthopaedic Surgery and Research Epidural fibrosis Laminectomy TGF-β Biomaterials |
| title | Mechanical barriers and transforming growth factor beta inhibitor on epidural fibrosis in a rabbit laminectomy model |
| title_full | Mechanical barriers and transforming growth factor beta inhibitor on epidural fibrosis in a rabbit laminectomy model |
| title_fullStr | Mechanical barriers and transforming growth factor beta inhibitor on epidural fibrosis in a rabbit laminectomy model |
| title_full_unstemmed | Mechanical barriers and transforming growth factor beta inhibitor on epidural fibrosis in a rabbit laminectomy model |
| title_short | Mechanical barriers and transforming growth factor beta inhibitor on epidural fibrosis in a rabbit laminectomy model |
| title_sort | mechanical barriers and transforming growth factor beta inhibitor on epidural fibrosis in a rabbit laminectomy model |
| topic | Epidural fibrosis Laminectomy TGF-β Biomaterials |
| url | http://link.springer.com/article/10.1186/s13018-018-0781-6 |
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