Studying the Geroprotective Properties of YAP/TAZ Signaling Inhibitors on <i>Drosophila melanogaster</i> Model
The transcriptional coactivators Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ) are the main downstream effectors of the evolutionarily conserved Hippo signaling pathway. YAP/TAZ are implicated in the transcriptional regulation of target genes that are invo...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-03-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/6/6006 |
| _version_ | 1797611204890329088 |
|---|---|
| author | Denis A. Golubev Nadezhda V. Zemskaya Anastasia A. Gorbunova Daria V. Kukuman Alexey Moskalev Mikhail V. Shaposhnikov |
| author_facet | Denis A. Golubev Nadezhda V. Zemskaya Anastasia A. Gorbunova Daria V. Kukuman Alexey Moskalev Mikhail V. Shaposhnikov |
| author_sort | Denis A. Golubev |
| collection | DOAJ |
| description | The transcriptional coactivators Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ) are the main downstream effectors of the evolutionarily conserved Hippo signaling pathway. YAP/TAZ are implicated in the transcriptional regulation of target genes that are involved in a wide range of key biological processes affecting tissue homeostasis and play dual roles in the aging process, depending on the cellular and tissue context. The aim of the present study was to investigate whether pharmacological inhibitors of Yap/Taz increase the lifespan of <i>Drosophila melanogaster</i>. Real-time qRT-PCR was performed to measure the changes in the expression of <i>Yki</i> (<i>Yorkie</i>, the <i>Drosophila</i> homolog of YAP/TAZ) target genes. We have revealed a lifespan-increasing effect of YAP/TAZ inhibitors that was mostly associated with decreased expression levels of the <i>wg</i> and <i>E2f1</i> genes. However, further analysis is required to understand the link between the YAP/TAZ pathway and aging. |
| first_indexed | 2024-03-11T06:24:32Z |
| format | Article |
| id | doaj.art-e3005db5ef9145f799b0f8911ca95a0d |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T06:24:32Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-e3005db5ef9145f799b0f8911ca95a0d2023-11-17T11:42:12ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01246600610.3390/ijms24066006Studying the Geroprotective Properties of YAP/TAZ Signaling Inhibitors on <i>Drosophila melanogaster</i> ModelDenis A. Golubev0Nadezhda V. Zemskaya1Anastasia A. Gorbunova2Daria V. Kukuman3Alexey Moskalev4Mikhail V. Shaposhnikov5Laboratory of Geroprotective and Radioprotective Technologies, Institute of Biology, Komi Science Center, Ural Branch, Russian Academy of Sciences, 167982 Syktyvkar, RussiaLaboratory of Geroprotective and Radioprotective Technologies, Institute of Biology, Komi Science Center, Ural Branch, Russian Academy of Sciences, 167982 Syktyvkar, RussiaLaboratory of Geroprotective and Radioprotective Technologies, Institute of Biology, Komi Science Center, Ural Branch, Russian Academy of Sciences, 167982 Syktyvkar, RussiaLaboratory of Geroprotective and Radioprotective Technologies, Institute of Biology, Komi Science Center, Ural Branch, Russian Academy of Sciences, 167982 Syktyvkar, RussiaLaboratory of Geroprotective and Radioprotective Technologies, Institute of Biology, Komi Science Center, Ural Branch, Russian Academy of Sciences, 167982 Syktyvkar, RussiaLaboratory of Geroprotective and Radioprotective Technologies, Institute of Biology, Komi Science Center, Ural Branch, Russian Academy of Sciences, 167982 Syktyvkar, RussiaThe transcriptional coactivators Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ) are the main downstream effectors of the evolutionarily conserved Hippo signaling pathway. YAP/TAZ are implicated in the transcriptional regulation of target genes that are involved in a wide range of key biological processes affecting tissue homeostasis and play dual roles in the aging process, depending on the cellular and tissue context. The aim of the present study was to investigate whether pharmacological inhibitors of Yap/Taz increase the lifespan of <i>Drosophila melanogaster</i>. Real-time qRT-PCR was performed to measure the changes in the expression of <i>Yki</i> (<i>Yorkie</i>, the <i>Drosophila</i> homolog of YAP/TAZ) target genes. We have revealed a lifespan-increasing effect of YAP/TAZ inhibitors that was mostly associated with decreased expression levels of the <i>wg</i> and <i>E2f1</i> genes. However, further analysis is required to understand the link between the YAP/TAZ pathway and aging.https://www.mdpi.com/1422-0067/24/6/6006YAP/TAZ<i>Drosophila</i>aginggeroprotector |
| spellingShingle | Denis A. Golubev Nadezhda V. Zemskaya Anastasia A. Gorbunova Daria V. Kukuman Alexey Moskalev Mikhail V. Shaposhnikov Studying the Geroprotective Properties of YAP/TAZ Signaling Inhibitors on <i>Drosophila melanogaster</i> Model International Journal of Molecular Sciences YAP/TAZ <i>Drosophila</i> aging geroprotector |
| title | Studying the Geroprotective Properties of YAP/TAZ Signaling Inhibitors on <i>Drosophila melanogaster</i> Model |
| title_full | Studying the Geroprotective Properties of YAP/TAZ Signaling Inhibitors on <i>Drosophila melanogaster</i> Model |
| title_fullStr | Studying the Geroprotective Properties of YAP/TAZ Signaling Inhibitors on <i>Drosophila melanogaster</i> Model |
| title_full_unstemmed | Studying the Geroprotective Properties of YAP/TAZ Signaling Inhibitors on <i>Drosophila melanogaster</i> Model |
| title_short | Studying the Geroprotective Properties of YAP/TAZ Signaling Inhibitors on <i>Drosophila melanogaster</i> Model |
| title_sort | studying the geroprotective properties of yap taz signaling inhibitors on i drosophila melanogaster i model |
| topic | YAP/TAZ <i>Drosophila</i> aging geroprotector |
| url | https://www.mdpi.com/1422-0067/24/6/6006 |
| work_keys_str_mv | AT denisagolubev studyingthegeroprotectivepropertiesofyaptazsignalinginhibitorsonidrosophilamelanogasterimodel AT nadezhdavzemskaya studyingthegeroprotectivepropertiesofyaptazsignalinginhibitorsonidrosophilamelanogasterimodel AT anastasiaagorbunova studyingthegeroprotectivepropertiesofyaptazsignalinginhibitorsonidrosophilamelanogasterimodel AT dariavkukuman studyingthegeroprotectivepropertiesofyaptazsignalinginhibitorsonidrosophilamelanogasterimodel AT alexeymoskalev studyingthegeroprotectivepropertiesofyaptazsignalinginhibitorsonidrosophilamelanogasterimodel AT mikhailvshaposhnikov studyingthegeroprotectivepropertiesofyaptazsignalinginhibitorsonidrosophilamelanogasterimodel |