Extract of Cimicifuga racemosa (L.) Nutt protects ovarian follicle reserve of mice against in vitro deleterious effects of dexamethasone
The present study aims to investigate if Cimicifuga racemosa (L.) Nutt extract (CIMI) reduces deleterious effects of dexamethasone (DEXA) in ovaries cultured in vitro. Mouse ovaries were collected and cultured in DMEM+ only or supplemented with 5 ng/mL of CIMI, or 4 ng/mL DEXA, or both CIMI and DEXA...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2023-09-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2023000100667&tlng=en |
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| author | E.I.T. de Assis V.A.N. Azevedo M.F. de Lima Neto F.C. Costa L.R.F.M. Paulino P.A.A. Barroso M.H.T. Matos A.P.O. do Monte M.A.M. Donato C.A. Peixoto A.N. Godinho J.M.O. Freire A.L.P. Souza J.R.V. Silva A.W.B. Silva |
| author_facet | E.I.T. de Assis V.A.N. Azevedo M.F. de Lima Neto F.C. Costa L.R.F.M. Paulino P.A.A. Barroso M.H.T. Matos A.P.O. do Monte M.A.M. Donato C.A. Peixoto A.N. Godinho J.M.O. Freire A.L.P. Souza J.R.V. Silva A.W.B. Silva |
| author_sort | E.I.T. de Assis |
| collection | DOAJ |
| description | The present study aims to investigate if Cimicifuga racemosa (L.) Nutt extract (CIMI) reduces deleterious effects of dexamethasone (DEXA) in ovaries cultured in vitro. Mouse ovaries were collected and cultured in DMEM+ only or supplemented with 5 ng/mL of CIMI, or 4 ng/mL DEXA, or both CIMI and DEXA. The ovaries were cultured at 37.5°C in 5% CO2 for 6 days. Ovarian morphology, follicular ultrastructure, and the levels of mRNA for Bax, Bcl-2, and Caspase-3 were evaluated. The results showed that DEXA reduced the percentage of morphologically normal follicles, while CIMI prevented the deleterious effects caused by DEXA. In addition, DEXA negatively affected the stromal cellular density, while CIMI prevented these adverse effects. Ovaries cultured with DEXA and CIMI showed similar levels of mRNA for Bax, Bcl-2, and Caspase-3 compared to those cultured in control medium, while ovaries cultured with DEXA had increased expression of the above genes. Additionally, the ultrastructure of the ovaries cultured with CIMI was well preserved. Thus, the extract of CIMI was able to prevent the deleterious effects caused by DEXA on cultured mouse ovaries. |
| first_indexed | 2024-03-11T21:53:07Z |
| format | Article |
| id | doaj.art-e302348ce4cc469895986e40fbee6e98 |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-03-11T21:53:07Z |
| publishDate | 2023-09-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-e302348ce4cc469895986e40fbee6e982023-09-26T07:40:54ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2023-09-015610.1590/1414-431x2023e12811Extract of Cimicifuga racemosa (L.) Nutt protects ovarian follicle reserve of mice against in vitro deleterious effects of dexamethasoneE.I.T. de Assishttps://orcid.org/0000-0001-8164-3937V.A.N. Azevedohttps://orcid.org/0000-0003-3194-8913M.F. de Lima Netohttps://orcid.org/0000-0003-0034-0606F.C. Costahttps://orcid.org/0000-0002-7045-1523L.R.F.M. Paulinohttps://orcid.org/0000-0002-4750-3436P.A.A. Barrosohttps://orcid.org/0000-0002-0243-7471M.H.T. Matoshttps://orcid.org/0000-0001-6215-5292A.P.O. do Montehttps://orcid.org/0000-0001-9945-4552M.A.M. Donatohttps://orcid.org/0000-0002-3361-2789C.A. Peixotohttps://orcid.org/0000-0002-7008-5930A.N. Godinhohttps://orcid.org/0000-0003-3410-2315J.M.O. Freirehttps://orcid.org/0000-0002-3412-8964A.L.P. Souzahttps://orcid.org/0000-0002-0348-4026J.R.V. Silvahttps://orcid.org/0000-0002-5970-6177A.W.B. Silvahttps://orcid.org/0000-0003-4105-426XThe present study aims to investigate if Cimicifuga racemosa (L.) Nutt extract (CIMI) reduces deleterious effects of dexamethasone (DEXA) in ovaries cultured in vitro. Mouse ovaries were collected and cultured in DMEM+ only or supplemented with 5 ng/mL of CIMI, or 4 ng/mL DEXA, or both CIMI and DEXA. The ovaries were cultured at 37.5°C in 5% CO2 for 6 days. Ovarian morphology, follicular ultrastructure, and the levels of mRNA for Bax, Bcl-2, and Caspase-3 were evaluated. The results showed that DEXA reduced the percentage of morphologically normal follicles, while CIMI prevented the deleterious effects caused by DEXA. In addition, DEXA negatively affected the stromal cellular density, while CIMI prevented these adverse effects. Ovaries cultured with DEXA and CIMI showed similar levels of mRNA for Bax, Bcl-2, and Caspase-3 compared to those cultured in control medium, while ovaries cultured with DEXA had increased expression of the above genes. Additionally, the ultrastructure of the ovaries cultured with CIMI was well preserved. Thus, the extract of CIMI was able to prevent the deleterious effects caused by DEXA on cultured mouse ovaries.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2023000100667&tlng=enFolliculogenesisToxicityMiceGlucocorticoidIn vitro culture |
| spellingShingle | E.I.T. de Assis V.A.N. Azevedo M.F. de Lima Neto F.C. Costa L.R.F.M. Paulino P.A.A. Barroso M.H.T. Matos A.P.O. do Monte M.A.M. Donato C.A. Peixoto A.N. Godinho J.M.O. Freire A.L.P. Souza J.R.V. Silva A.W.B. Silva Extract of Cimicifuga racemosa (L.) Nutt protects ovarian follicle reserve of mice against in vitro deleterious effects of dexamethasone Brazilian Journal of Medical and Biological Research Folliculogenesis Toxicity Mice Glucocorticoid In vitro culture |
| title | Extract of Cimicifuga racemosa (L.) Nutt protects ovarian follicle reserve of mice against in vitro deleterious effects of dexamethasone |
| title_full | Extract of Cimicifuga racemosa (L.) Nutt protects ovarian follicle reserve of mice against in vitro deleterious effects of dexamethasone |
| title_fullStr | Extract of Cimicifuga racemosa (L.) Nutt protects ovarian follicle reserve of mice against in vitro deleterious effects of dexamethasone |
| title_full_unstemmed | Extract of Cimicifuga racemosa (L.) Nutt protects ovarian follicle reserve of mice against in vitro deleterious effects of dexamethasone |
| title_short | Extract of Cimicifuga racemosa (L.) Nutt protects ovarian follicle reserve of mice against in vitro deleterious effects of dexamethasone |
| title_sort | extract of cimicifuga racemosa l nutt protects ovarian follicle reserve of mice against in vitro deleterious effects of dexamethasone |
| topic | Folliculogenesis Toxicity Mice Glucocorticoid In vitro culture |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2023000100667&tlng=en |
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