Fresh and cultured buccal cells as a source of mRNA and protein for molecular analysis
We developed a method for obtaining viable buccal cells from mouthwash samples for use as a source of mRNA and protein. Immunofluorescent analysis showed that most cells were derived from nonkeratinized parabasal epithelia, with a minor proportion of proliferative cells. Gene expression was detected...
मुख्य लेखकों: | , , , |
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स्वरूप: | लेख |
भाषा: | English |
प्रकाशित: |
Taylor & Francis Group
2004-08-01
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श्रृंखला: | BioTechniques |
ऑनलाइन पहुंच: | https://www.future-science.com/doi/10.2144/04372RR03 |