Protocol for quantification of UDP-GlcNAc using an enzymatic microplate assay
Summary: Uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) is the end product of the hexosamine biosynthetic pathway and the substrate for protein O-linked N-acetylglucosaminylation (O-GlcNAcylation). Here, we present a protocol for the quantification of UDP-GlcNAc using an enzymatic microplate a...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2024-03-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723007840 |