Protocol for quantification of UDP-GlcNAc using an enzymatic microplate assay

Protocol for quantification of UDP-GlcNAc using an enzymatic microplate assay

Summary: Uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) is the end product of the hexosamine biosynthetic pathway and the substrate for protein O-linked N-acetylglucosaminylation (O-GlcNAcylation). Here, we present a protocol for the quantification of UDP-GlcNAc using an enzymatic microplate a...

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Detalles Bibliográficos
Autores principales: Divya Upadhyay, Rishi Banerjee, Jukka Kallijärvi, Janne Purhonen
Formato: Artículo
Lenguaje:English
Publicado: Elsevier 2024-03-01
Colección:STAR Protocols
Materias:
Cell Biology
Metabolism
Molecular Biology
Acceso en línea:http://www.sciencedirect.com/science/article/pii/S2666166723007840

Internet

http://www.sciencedirect.com/science/article/pii/S2666166723007840

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