Targeted removal of mitochondrial DNA from mouse and human extrachromosomal circular DNA with CRISPR-Cas9

Targeted removal of mitochondrial DNA from mouse and human extrachromosomal circular DNA with CRISPR-Cas9

Extrachromosomal circular DNA (eccDNA) of chromosomal origin is common in eukaryotic cells. Amplification of oncogenes on large eccDNA (ecDNA) can drive biological processes such as tumorigenesis, and identification of eccDNA by sequencing after removal of chromosomal DNA is therefore important for...

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Bibliographic Details
Main Authors: Weijia Feng, Gerard Arrey, Egija Zole, Wei lv, Xue Liang, Peng Han, Marghoob Mohiyuddin, Henriette Pilegaard, Birgitte Regenberg
Format: Article
Language:English
Published: Elsevier 2022-01-01
Series:Computational and Structural Biotechnology Journal
Subjects:
mtDNA removal
CRISPR/Cas9
eccDNA
ecDNA
Online Access:http://www.sciencedirect.com/science/article/pii/S2001037022002392

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http://www.sciencedirect.com/science/article/pii/S2001037022002392

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