Internal and external normalization of nascent RNA sequencing run-on experiments
Abstract In experiments with significant perturbations to transcription, nascent RNA sequencing protocols are dependent on external spike-ins for reliable normalization. Unlike in RNA-seq, these spike-ins are not standardized and, in many cases, depend on a run-on reaction that is assumed to have co...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-01-01
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| Series: | BMC Bioinformatics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12859-023-05607-3 |