Knock down of target genes by RNA interference in the embryos of lepidopteran insect, Bombyx mori

Summary: RNA interference (RNAi) is a technique used for posttranscriptional gene silencing, but lepidopteran insects are not sensitive to RNAi. Here, we present a protocol for knocking down the expression level of target genes by RNAi in Bombyx mori embryos. We describe the preparation of double-st...

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Bibliographic Details
Main Authors: Guanfeng Xu, Yulin Tian, Yuling Peng, Sichun Zheng
Format: Article
Language:English
Published: Elsevier 2022-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722000995