Knock down of target genes by RNA interference in the embryos of lepidopteran insect, Bombyx mori
Summary: RNA interference (RNAi) is a technique used for posttranscriptional gene silencing, but lepidopteran insects are not sensitive to RNAi. Here, we present a protocol for knocking down the expression level of target genes by RNAi in Bombyx mori embryos. We describe the preparation of double-st...
Main Authors: | Guanfeng Xu, Yulin Tian, Yuling Peng, Sichun Zheng |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2022-03-01
|
Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722000995 |
Similar Items
-
Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila
by: J. Eduardo Pérez-Mojica, et al.
Published: (2023-09-01) -
Biased embryos and evolution /
by: 177575 Arthur, Wallace
Published: (2004) -
Visualizing and manipulating the production and accumulation of hyaluronan for functional assessment in chicken embryos
by: Bhargav D. Sanketi, et al.
Published: (2023-06-01) -
Depletion of nuclear LINE1 RNA in mouse ESCs and embryos
by: Michelle Percharde, et al.
Published: (2021-09-01) -
A protocol for in ovo electroporation of chicken and snake embryos to study forebrain development
by: Adrián Cárdenas, et al.
Published: (2021-09-01)