Antimutagenic Properties of Basil (Ocimum basilicum L.) in Salmonella typhimurium TA100
The use of dietary antimutagens and anticarcinogens has been seen as a promising approach to the protection of human health. Basil (Ocimum basilicum L.) is a well-known medicinal and aromatic plant, with a range of newly discovered biological activities possibly important for chemoprevention. In the...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Zagreb Faculty of Food Technology and Biotechnology
2007-01-01
|
| Series: | Food Technology and Biotechnology |
| Subjects: | |
| Online Access: | http://hrcak.srce.hr/file/43789 |
| _version_ | 1827614910201200640 |
|---|---|
| author | Olivera Stajković Dragana Mitić-Ćulafić Slaviša Stanković Branka Vuković-Gačić Draga Simić Jelena Knežević-Vukčević Tanja Berić-Bjedov |
| author_facet | Olivera Stajković Dragana Mitić-Ćulafić Slaviša Stanković Branka Vuković-Gačić Draga Simić Jelena Knežević-Vukčević Tanja Berić-Bjedov |
| author_sort | Olivera Stajković |
| collection | DOAJ |
| description | The use of dietary antimutagens and anticarcinogens has been seen as a promising approach to the protection of human health. Basil (Ocimum basilicum L.) is a well-known medicinal and aromatic plant, with a range of newly discovered biological activities possibly important for chemoprevention. In the preliminary experiments, toxic and mutagenic potential of essential oil (EO) from basil and pure substances: linalool, ß-myrcene and 1,8-cineole were tested using Salmonella typhimurium TA98, TA100 and TA102, with and without S9 mix (microsomal fraction of rat liver). No mutagenic effect of basil derivatives was detected in any tested strain. Antimutagenic effects of essential oil from basil and its pure constituents were further evaluated in the Ames test using S. typhimurium TA100. UVC irradiation and three chemical mutagens, 4-nitroquinoline-N-oxide (4NQO), 2-nitropropane (2-NP) and benzo(a)pyrene (B(a)P) were used to induce mutagenesis. All tested basil derivatives significantly reduced UV-induced mutations. The maximum inhibition was in the range of 64–77 %. Inhibitory potential against direct acting model mutagen/carcinogen 4NQO was similar to UV (52–67 %). In the presence of S9, EO and 1,8-cineole showed moderate inhibition of 2-NP induced mutagenesis, while the remaining two substances had no effect. Linalool exhibited high co-mutagenic effect with B(a)P, 1,8-cineole showed moderate inhibitory effect against B(a)P-induced mutations, while EO and ß-myrcene were ineffective. |
| first_indexed | 2024-03-09T09:01:37Z |
| format | Article |
| id | doaj.art-e3515e0ca77145d1933dbe6e2c63e550 |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-03-09T09:01:37Z |
| publishDate | 2007-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-e3515e0ca77145d1933dbe6e2c63e5502023-12-02T11:27:44ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062007-01-01452213217Antimutagenic Properties of Basil (Ocimum basilicum L.) in Salmonella typhimurium TA100Olivera Stajković0Dragana Mitić-Ćulafić1Slaviša Stanković2Branka Vuković-Gačić3Draga Simić4Jelena Knežević-Vukčević5Tanja Berić-Bjedov6Laboratory for Microbiology, Institute of Soil Science, Teodora Drajzera 7, 11000 Belgrade, SerbiaLaboratory for Microbiology, Faculty of Biology, University of Belgrade, Studentski trg 16,11000 Belgrade, SerbiaLaboratory for Microbiology, Faculty of Biology, University of Belgrade, Studentski trg 16,11000 Belgrade, SerbiaLaboratory for Microbiology, Faculty of Biology, University of Belgrade, Studentski trg 16,11000 Belgrade, SerbiaLaboratory for Microbiology, Faculty of Biology, University of Belgrade, Studentski trg 16,11000 Belgrade, SerbiaLaboratory for Microbiology, Faculty of Biology, University of Belgrade, Studentski trg 16,11000 Belgrade, SerbiaLaboratory for Microbiology, Faculty of Biology, University of Belgrade, Studentski trg 16,11000 Belgrade, SerbiaThe use of dietary antimutagens and anticarcinogens has been seen as a promising approach to the protection of human health. Basil (Ocimum basilicum L.) is a well-known medicinal and aromatic plant, with a range of newly discovered biological activities possibly important for chemoprevention. In the preliminary experiments, toxic and mutagenic potential of essential oil (EO) from basil and pure substances: linalool, ß-myrcene and 1,8-cineole were tested using Salmonella typhimurium TA98, TA100 and TA102, with and without S9 mix (microsomal fraction of rat liver). No mutagenic effect of basil derivatives was detected in any tested strain. Antimutagenic effects of essential oil from basil and its pure constituents were further evaluated in the Ames test using S. typhimurium TA100. UVC irradiation and three chemical mutagens, 4-nitroquinoline-N-oxide (4NQO), 2-nitropropane (2-NP) and benzo(a)pyrene (B(a)P) were used to induce mutagenesis. All tested basil derivatives significantly reduced UV-induced mutations. The maximum inhibition was in the range of 64–77 %. Inhibitory potential against direct acting model mutagen/carcinogen 4NQO was similar to UV (52–67 %). In the presence of S9, EO and 1,8-cineole showed moderate inhibition of 2-NP induced mutagenesis, while the remaining two substances had no effect. Linalool exhibited high co-mutagenic effect with B(a)P, 1,8-cineole showed moderate inhibitory effect against B(a)P-induced mutations, while EO and ß-myrcene were ineffective.http://hrcak.srce.hr/file/437894-nitroquinoline-N-oxide (4NQO)2-nitropropane (2-NP)benzo(a)pyrene (B(a)P)UVC irradiationOcimum basilicumSalmonella typhimurium TA100 |
| spellingShingle | Olivera Stajković Dragana Mitić-Ćulafić Slaviša Stanković Branka Vuković-Gačić Draga Simić Jelena Knežević-Vukčević Tanja Berić-Bjedov Antimutagenic Properties of Basil (Ocimum basilicum L.) in Salmonella typhimurium TA100 Food Technology and Biotechnology 4-nitroquinoline-N-oxide (4NQO) 2-nitropropane (2-NP) benzo(a)pyrene (B(a)P) UVC irradiation Ocimum basilicum Salmonella typhimurium TA100 |
| title | Antimutagenic Properties of Basil (Ocimum basilicum L.) in Salmonella typhimurium TA100 |
| title_full | Antimutagenic Properties of Basil (Ocimum basilicum L.) in Salmonella typhimurium TA100 |
| title_fullStr | Antimutagenic Properties of Basil (Ocimum basilicum L.) in Salmonella typhimurium TA100 |
| title_full_unstemmed | Antimutagenic Properties of Basil (Ocimum basilicum L.) in Salmonella typhimurium TA100 |
| title_short | Antimutagenic Properties of Basil (Ocimum basilicum L.) in Salmonella typhimurium TA100 |
| title_sort | antimutagenic properties of basil ocimum basilicum l in salmonella typhimurium ta100 |
| topic | 4-nitroquinoline-N-oxide (4NQO) 2-nitropropane (2-NP) benzo(a)pyrene (B(a)P) UVC irradiation Ocimum basilicum Salmonella typhimurium TA100 |
| url | http://hrcak.srce.hr/file/43789 |
| work_keys_str_mv | AT oliverastajkovic antimutagenicpropertiesofbasilocimumbasilicumlinsalmonellatyphimuriumta100 AT draganamiticculafic antimutagenicpropertiesofbasilocimumbasilicumlinsalmonellatyphimuriumta100 AT slavisastankovic antimutagenicpropertiesofbasilocimumbasilicumlinsalmonellatyphimuriumta100 AT brankavukovicgacic antimutagenicpropertiesofbasilocimumbasilicumlinsalmonellatyphimuriumta100 AT dragasimic antimutagenicpropertiesofbasilocimumbasilicumlinsalmonellatyphimuriumta100 AT jelenaknezevicvukcevic antimutagenicpropertiesofbasilocimumbasilicumlinsalmonellatyphimuriumta100 AT tanjabericbjedov antimutagenicpropertiesofbasilocimumbasilicumlinsalmonellatyphimuriumta100 |