Nutrition Related Stress Factors Reduce the Transfer of Extended-Spectrum Beta-Lactamase Resistance Genes between an <i>Escherichia coli</i> Donor and a <i>Salmonella</i> Typhimurium Recipient In Vitro
The transfer of extended spectrum β-lactamase (ESBL)-genes occurs frequently between different bacteria species. The aim of this study was to investigate the impact of nutrition related stress factors on this transfer. Thus, an <i>Escherichia coli</i> donor and a <i>Salmon...
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MDPI AG
2019-07-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/9/8/324 |
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| author | Eva-Maria Saliu Marita Eitinger Jürgen Zentek Wilfried Vahjen |
| author_facet | Eva-Maria Saliu Marita Eitinger Jürgen Zentek Wilfried Vahjen |
| author_sort | Eva-Maria Saliu |
| collection | DOAJ |
| description | The transfer of extended spectrum β-lactamase (ESBL)-genes occurs frequently between different bacteria species. The aim of this study was to investigate the impact of nutrition related stress factors on this transfer. Thus, an <i>Escherichia coli</i> donor and a <i>Salmonella</i> Typhimurium recipient were co-incubated for 4 h in media containing different levels of the stress factors’ pH, osmolality, copper, zinc and acetic, propionic, lactic, and n-butyric acid, as well as subtherapeutic levels of cefotaxime, sulfamethoxazole/trimethoprim, and nitrofurantoin. Conjugation frequencies were calculated as transconjugants per donor, recipient, and total bacterial count. A correction factor for the stress impact on bacterial growth was used. Acetic, lactic, and n-butyric, acid, as well as pH, showed no significant impact. In contrast, increasing concentrations of propionate, zinc, copper, and nitrofurantoin, as well as increased osmolality reduced conjugation frequencies. Sulfamethoxazole/trimethoprim and cefotaxime showed increased transconjugants per donor, which decreased after correction for stress. This study showed, for the model mating pair, that conjugation frequencies decreased under different physiological stress conditions, and, thus, the hypothesis that stress factors may enhance conjugation should be viewed with caution. Furthermore, for studies on in vitro gene transfer, it is vital to consider the impact of studied stressors on bacterial growth. |
| first_indexed | 2024-12-13T10:07:33Z |
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| id | doaj.art-e3555d58c1d14c92ab67a57f8fd885ac |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-12-13T10:07:33Z |
| publishDate | 2019-07-01 |
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| series | Biomolecules |
| spelling | doaj.art-e3555d58c1d14c92ab67a57f8fd885ac2022-12-21T23:51:31ZengMDPI AGBiomolecules2218-273X2019-07-019832410.3390/biom9080324biom9080324Nutrition Related Stress Factors Reduce the Transfer of Extended-Spectrum Beta-Lactamase Resistance Genes between an <i>Escherichia coli</i> Donor and a <i>Salmonella</i> Typhimurium Recipient In VitroEva-Maria Saliu0Marita Eitinger1Jürgen Zentek2Wilfried Vahjen3Freie Universität Berlin, Institute of Animal Nutrition, Königin-Luise-Str. 49, 14195 Berlin, GermanyFreie Universität Berlin, Institute of Animal Nutrition, Königin-Luise-Str. 49, 14195 Berlin, GermanyFreie Universität Berlin, Institute of Animal Nutrition, Königin-Luise-Str. 49, 14195 Berlin, GermanyFreie Universität Berlin, Institute of Animal Nutrition, Königin-Luise-Str. 49, 14195 Berlin, GermanyThe transfer of extended spectrum β-lactamase (ESBL)-genes occurs frequently between different bacteria species. The aim of this study was to investigate the impact of nutrition related stress factors on this transfer. Thus, an <i>Escherichia coli</i> donor and a <i>Salmonella</i> Typhimurium recipient were co-incubated for 4 h in media containing different levels of the stress factors’ pH, osmolality, copper, zinc and acetic, propionic, lactic, and n-butyric acid, as well as subtherapeutic levels of cefotaxime, sulfamethoxazole/trimethoprim, and nitrofurantoin. Conjugation frequencies were calculated as transconjugants per donor, recipient, and total bacterial count. A correction factor for the stress impact on bacterial growth was used. Acetic, lactic, and n-butyric, acid, as well as pH, showed no significant impact. In contrast, increasing concentrations of propionate, zinc, copper, and nitrofurantoin, as well as increased osmolality reduced conjugation frequencies. Sulfamethoxazole/trimethoprim and cefotaxime showed increased transconjugants per donor, which decreased after correction for stress. This study showed, for the model mating pair, that conjugation frequencies decreased under different physiological stress conditions, and, thus, the hypothesis that stress factors may enhance conjugation should be viewed with caution. Furthermore, for studies on in vitro gene transfer, it is vital to consider the impact of studied stressors on bacterial growth.https://www.mdpi.com/2218-273X/9/8/324extended-spectrum β-lactamaseshorizontal gene transfermineralsshort-chain fatty acidsorganic acidsfeed additivesosmolaritybacterial conjugation |
| spellingShingle | Eva-Maria Saliu Marita Eitinger Jürgen Zentek Wilfried Vahjen Nutrition Related Stress Factors Reduce the Transfer of Extended-Spectrum Beta-Lactamase Resistance Genes between an <i>Escherichia coli</i> Donor and a <i>Salmonella</i> Typhimurium Recipient In Vitro Biomolecules extended-spectrum β-lactamases horizontal gene transfer minerals short-chain fatty acids organic acids feed additives osmolarity bacterial conjugation |
| title | Nutrition Related Stress Factors Reduce the Transfer of Extended-Spectrum Beta-Lactamase Resistance Genes between an <i>Escherichia coli</i> Donor and a <i>Salmonella</i> Typhimurium Recipient In Vitro |
| title_full | Nutrition Related Stress Factors Reduce the Transfer of Extended-Spectrum Beta-Lactamase Resistance Genes between an <i>Escherichia coli</i> Donor and a <i>Salmonella</i> Typhimurium Recipient In Vitro |
| title_fullStr | Nutrition Related Stress Factors Reduce the Transfer of Extended-Spectrum Beta-Lactamase Resistance Genes between an <i>Escherichia coli</i> Donor and a <i>Salmonella</i> Typhimurium Recipient In Vitro |
| title_full_unstemmed | Nutrition Related Stress Factors Reduce the Transfer of Extended-Spectrum Beta-Lactamase Resistance Genes between an <i>Escherichia coli</i> Donor and a <i>Salmonella</i> Typhimurium Recipient In Vitro |
| title_short | Nutrition Related Stress Factors Reduce the Transfer of Extended-Spectrum Beta-Lactamase Resistance Genes between an <i>Escherichia coli</i> Donor and a <i>Salmonella</i> Typhimurium Recipient In Vitro |
| title_sort | nutrition related stress factors reduce the transfer of extended spectrum beta lactamase resistance genes between an i escherichia coli i donor and a i salmonella i typhimurium recipient in vitro |
| topic | extended-spectrum β-lactamases horizontal gene transfer minerals short-chain fatty acids organic acids feed additives osmolarity bacterial conjugation |
| url | https://www.mdpi.com/2218-273X/9/8/324 |
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