α-Glucosidase Inhibitory Activity of Fermented Okara Broth Started with the Strain <i>Bacillus amyloliquefaciens</i> SY07
In this work, a new strain of <i>Bacillus amyloliquefaciens</i> SY07 isolated from a traditional fermented soybean food was reported to possess remarkable α-glucosidase inhibitor-producing ability. Different culture media were applied for the proliferation of <i>B.</i> amylol...
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| Format: | Article |
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MDPI AG
2022-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/3/1127 |
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| author | Yifang Gao Wenhui Bian Yuanyuan Fang Peng Du Xueting Liu Xueru Zhao Fengjuan Li |
| author_facet | Yifang Gao Wenhui Bian Yuanyuan Fang Peng Du Xueting Liu Xueru Zhao Fengjuan Li |
| author_sort | Yifang Gao |
| collection | DOAJ |
| description | In this work, a new strain of <i>Bacillus amyloliquefaciens</i> SY07 isolated from a traditional fermented soybean food was reported to possess remarkable α-glucosidase inhibitor-producing ability. Different culture media were applied for the proliferation of <i>B.</i> amyloliquefaciens SY07, and it was found that fermented okara broth presented the highest α-glucosidase inhibitory activity, while Luria-Bertani medium showed a negative effect. The extract from fermented okara broth acted in a dose-dependent manner to inhibit α-glucosidase activity, with an IC<sub>50</sub> value of 0.454 mg/mL, and main inhibitors in the fermentation extract presented a reversible, uncompetitive pattern according to Lineweaver–Burk plots. Moreover, 1-deoxynojirimycin, a recognized α-glucosidase inhibitor, was found in the extract. Results indicated that <i>B. amyloliquefaciens</i> SY07 could utilize okara, a by-product from the soy processing industry, to generate α-glucosidase inhibitors effectively, and be regarded as a novel excellent microbial candidate for safe, economical production of potential functional foods or ingredients with hypoglycemic effect. |
| first_indexed | 2024-03-09T23:26:49Z |
| format | Article |
| id | doaj.art-e36a9d117d254941a6a8fd47da28b4bd |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T23:26:49Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-e36a9d117d254941a6a8fd47da28b4bd2023-11-23T17:18:07ZengMDPI AGMolecules1420-30492022-02-01273112710.3390/molecules27031127α-Glucosidase Inhibitory Activity of Fermented Okara Broth Started with the Strain <i>Bacillus amyloliquefaciens</i> SY07Yifang Gao0Wenhui Bian1Yuanyuan Fang2Peng Du3Xueting Liu4Xueru Zhao5Fengjuan Li6Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Ministry of Education, No. 29 13th Avenue, Teda, Tianjin 300457, ChinaKey Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Ministry of Education, No. 29 13th Avenue, Teda, Tianjin 300457, ChinaKey Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Ministry of Education, No. 29 13th Avenue, Teda, Tianjin 300457, ChinaKey Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Ministry of Education, No. 29 13th Avenue, Teda, Tianjin 300457, ChinaKey Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Ministry of Education, No. 29 13th Avenue, Teda, Tianjin 300457, ChinaKey Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Ministry of Education, No. 29 13th Avenue, Teda, Tianjin 300457, ChinaKey Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Ministry of Education, No. 29 13th Avenue, Teda, Tianjin 300457, ChinaIn this work, a new strain of <i>Bacillus amyloliquefaciens</i> SY07 isolated from a traditional fermented soybean food was reported to possess remarkable α-glucosidase inhibitor-producing ability. Different culture media were applied for the proliferation of <i>B.</i> amyloliquefaciens SY07, and it was found that fermented okara broth presented the highest α-glucosidase inhibitory activity, while Luria-Bertani medium showed a negative effect. The extract from fermented okara broth acted in a dose-dependent manner to inhibit α-glucosidase activity, with an IC<sub>50</sub> value of 0.454 mg/mL, and main inhibitors in the fermentation extract presented a reversible, uncompetitive pattern according to Lineweaver–Burk plots. Moreover, 1-deoxynojirimycin, a recognized α-glucosidase inhibitor, was found in the extract. Results indicated that <i>B. amyloliquefaciens</i> SY07 could utilize okara, a by-product from the soy processing industry, to generate α-glucosidase inhibitors effectively, and be regarded as a novel excellent microbial candidate for safe, economical production of potential functional foods or ingredients with hypoglycemic effect.https://www.mdpi.com/1420-3049/27/3/1127<i>Bacillus amyloliquefaciens</i> SY07α-glucosidase inhibitory activity1-deoxynojirimycin (DNJ)okarainhibition kinetics |
| spellingShingle | Yifang Gao Wenhui Bian Yuanyuan Fang Peng Du Xueting Liu Xueru Zhao Fengjuan Li α-Glucosidase Inhibitory Activity of Fermented Okara Broth Started with the Strain <i>Bacillus amyloliquefaciens</i> SY07 Molecules <i>Bacillus amyloliquefaciens</i> SY07 α-glucosidase inhibitory activity 1-deoxynojirimycin (DNJ) okara inhibition kinetics |
| title | α-Glucosidase Inhibitory Activity of Fermented Okara Broth Started with the Strain <i>Bacillus amyloliquefaciens</i> SY07 |
| title_full | α-Glucosidase Inhibitory Activity of Fermented Okara Broth Started with the Strain <i>Bacillus amyloliquefaciens</i> SY07 |
| title_fullStr | α-Glucosidase Inhibitory Activity of Fermented Okara Broth Started with the Strain <i>Bacillus amyloliquefaciens</i> SY07 |
| title_full_unstemmed | α-Glucosidase Inhibitory Activity of Fermented Okara Broth Started with the Strain <i>Bacillus amyloliquefaciens</i> SY07 |
| title_short | α-Glucosidase Inhibitory Activity of Fermented Okara Broth Started with the Strain <i>Bacillus amyloliquefaciens</i> SY07 |
| title_sort | α glucosidase inhibitory activity of fermented okara broth started with the strain i bacillus amyloliquefaciens i sy07 |
| topic | <i>Bacillus amyloliquefaciens</i> SY07 α-glucosidase inhibitory activity 1-deoxynojirimycin (DNJ) okara inhibition kinetics |
| url | https://www.mdpi.com/1420-3049/27/3/1127 |
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