Identification of protein targets for the antidepressant effects of Kai-Xin-San in Chinese medicine using isobaric tags for relative and absolute quantitation
Kai-Xin-San consists of Ginseng Radix, Polygalae Radix, Acori Tatarinowii Rhizoma, and Poria at a ratio of 3:3:2:2. Kai-Xin-San has been widely used for the treatment of emotional disorders in China. However, no studies have identified the key proteins implicated in response to Kai-Xin-San treatment...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2020-01-01
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| Series: | Neural Regeneration Research |
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| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2020;volume=15;issue=2;spage=302;epage=310;aulast=Dong |
| _version_ | 1818294279634681856 |
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| author | Xian-Zhe Dong Dong-Xiao Wang Tian-Yi Zhang Xu Liu Ping Liu Yuan Hu |
| author_facet | Xian-Zhe Dong Dong-Xiao Wang Tian-Yi Zhang Xu Liu Ping Liu Yuan Hu |
| author_sort | Xian-Zhe Dong |
| collection | DOAJ |
| description | Kai-Xin-San consists of Ginseng Radix, Polygalae Radix, Acori Tatarinowii Rhizoma, and Poria at a ratio of 3:3:2:2. Kai-Xin-San has been widely used for the treatment of emotional disorders in China. However, no studies have identified the key proteins implicated in response to Kai-Xin-San treatment. In this study, rat models of chronic mild stress were established using different stress methods over 28 days. After 14 days of stress stimulation, rats received daily intragastric administrations of 600 mg/kg Kai-Xin-San. The sucrose preference test was used to determine depression-like behavior in rats, while isobaric tags were used for relative and absolute quantitation-based proteomics to identify altered proteins following Kai-Xin-San treatment. Kai-Xin-San treatment for 2 weeks noticeably improved depression-like behaviors in rats with chronic mild stress. We identified 33 differentially expressed proteins: 7 were upregulated and 26 were downregulated. Functional analysis showed that these differentially expressed proteins participate in synaptic plasticity, neurodevelopment, and neurogenesis. Our results indicate that Kai-Xin-San has an important role in regulating the key node proteins in the synaptic signaling network, and are helpful to better understand the mechanism of the antidepressive effects of Kai-Xin-San and to provide objective theoretical support for its clinical application. The study was approved by the Ethics Committee for Animal Research from the Chinese PLA General Hospital (approval No. X5-2016-07) on March 5, 2016. |
| first_indexed | 2024-12-13T03:29:14Z |
| format | Article |
| id | doaj.art-e3782563e71f463c93526b14ebdb1fd3 |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-12-13T03:29:14Z |
| publishDate | 2020-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-e3782563e71f463c93526b14ebdb1fd32022-12-22T00:01:13ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742020-01-0115230231010.4103/1673-5374.265555Identification of protein targets for the antidepressant effects of Kai-Xin-San in Chinese medicine using isobaric tags for relative and absolute quantitationXian-Zhe DongDong-Xiao WangTian-Yi ZhangXu LiuPing LiuYuan HuKai-Xin-San consists of Ginseng Radix, Polygalae Radix, Acori Tatarinowii Rhizoma, and Poria at a ratio of 3:3:2:2. Kai-Xin-San has been widely used for the treatment of emotional disorders in China. However, no studies have identified the key proteins implicated in response to Kai-Xin-San treatment. In this study, rat models of chronic mild stress were established using different stress methods over 28 days. After 14 days of stress stimulation, rats received daily intragastric administrations of 600 mg/kg Kai-Xin-San. The sucrose preference test was used to determine depression-like behavior in rats, while isobaric tags were used for relative and absolute quantitation-based proteomics to identify altered proteins following Kai-Xin-San treatment. Kai-Xin-San treatment for 2 weeks noticeably improved depression-like behaviors in rats with chronic mild stress. We identified 33 differentially expressed proteins: 7 were upregulated and 26 were downregulated. Functional analysis showed that these differentially expressed proteins participate in synaptic plasticity, neurodevelopment, and neurogenesis. Our results indicate that Kai-Xin-San has an important role in regulating the key node proteins in the synaptic signaling network, and are helpful to better understand the mechanism of the antidepressive effects of Kai-Xin-San and to provide objective theoretical support for its clinical application. The study was approved by the Ethics Committee for Animal Research from the Chinese PLA General Hospital (approval No. X5-2016-07) on March 5, 2016.http://www.nrronline.org/article.asp?issn=1673-5374;year=2020;volume=15;issue=2;spage=302;epage=310;aulast=Dongbrain-derived neurotrophic factor signal pathway; depression; isobaric tags for relative and absolute quantitation; Kai-Xin-San; neurogenesis; protein network; proteomics analysis; synaptic plasticity; traditional Chinese medicine |
| spellingShingle | Xian-Zhe Dong Dong-Xiao Wang Tian-Yi Zhang Xu Liu Ping Liu Yuan Hu Identification of protein targets for the antidepressant effects of Kai-Xin-San in Chinese medicine using isobaric tags for relative and absolute quantitation Neural Regeneration Research brain-derived neurotrophic factor signal pathway; depression; isobaric tags for relative and absolute quantitation; Kai-Xin-San; neurogenesis; protein network; proteomics analysis; synaptic plasticity; traditional Chinese medicine |
| title | Identification of protein targets for the antidepressant effects of Kai-Xin-San in Chinese medicine using isobaric tags for relative and absolute quantitation |
| title_full | Identification of protein targets for the antidepressant effects of Kai-Xin-San in Chinese medicine using isobaric tags for relative and absolute quantitation |
| title_fullStr | Identification of protein targets for the antidepressant effects of Kai-Xin-San in Chinese medicine using isobaric tags for relative and absolute quantitation |
| title_full_unstemmed | Identification of protein targets for the antidepressant effects of Kai-Xin-San in Chinese medicine using isobaric tags for relative and absolute quantitation |
| title_short | Identification of protein targets for the antidepressant effects of Kai-Xin-San in Chinese medicine using isobaric tags for relative and absolute quantitation |
| title_sort | identification of protein targets for the antidepressant effects of kai xin san in chinese medicine using isobaric tags for relative and absolute quantitation |
| topic | brain-derived neurotrophic factor signal pathway; depression; isobaric tags for relative and absolute quantitation; Kai-Xin-San; neurogenesis; protein network; proteomics analysis; synaptic plasticity; traditional Chinese medicine |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2020;volume=15;issue=2;spage=302;epage=310;aulast=Dong |
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