The effect of anthocyanin from Dioscorea alata L. after purification, identification on antioxidant capacity in mice
Abstract Increasing findings devote to searching for natural active compositions as additives to ameliorate health status. Anthocyanin, water‐soluble natural pigment, has been concerned due to its favorable antioxidant activity. In this study, we purified anthocyanin from Dioscorea alata L., identif...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-10-01
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| Series: | Food Science & Nutrition |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/fsn3.3547 |
| _version_ | 1797662552029659136 |
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| author | Pingfei Qiu Junpu Chen Junlong Wu Qin Wang Yanrong Hu Xiaochun Li Huiyu Shi Xuemei Wang |
| author_facet | Pingfei Qiu Junpu Chen Junlong Wu Qin Wang Yanrong Hu Xiaochun Li Huiyu Shi Xuemei Wang |
| author_sort | Pingfei Qiu |
| collection | DOAJ |
| description | Abstract Increasing findings devote to searching for natural active compositions as additives to ameliorate health status. Anthocyanin, water‐soluble natural pigment, has been concerned due to its favorable antioxidant activity. In this study, we purified anthocyanin from Dioscorea alata L., identified its compounds, and evaluated its antioxidant properties. The results indicated that the purity of anthocyanin increased to 39.59 ± 1.56%, 60.18 ± 1.97%, and 81.08 ± 1.97% after purification via AB‐8 macroporous resin, Sep‐Pak C18 solid phase, and LH‐20 Sephadex stepwise. Ultra‐performance liquid chromatography tandem mass spectrometer results indicated that paeoniflorin‐3,5‐O‐dihexoside, petunin‐3‐O‐feruloyl‐glucoside‐5‐O‐glucoside, cyanidin‐3‐O‐feruloyl glucoside‐5‐O‐glucoside, cyanidin‐3‐O‐sophoroside, and petunin‐3,5‐O‐dihexoside were the major compounds. The purified anthocyanin exhibited stronger antioxidant activity than the unpurified extract and ascorbic acid, whereas weaker than that of cyanidin‐3‐O‐glucoside in general, which was assessed using DPPH, ABTS, and Fe3+ reducing capacity methods. Moreover, the purified anthocyanin increased GSH‐Px, total antioxidant capacity, and superoxide dismutase activities and decreased malondialdehyde concentration on serum in mice after administering lipopolysaccharide for 24 h (p < .05). To summarize, the purified anthocyanin boasts more outstanding antioxidant properties than that of crude extracts. These results provide a reference with source of anthocyanin and it is conducive to use Dioscorea alata L. resources. |
| first_indexed | 2024-03-11T19:00:53Z |
| format | Article |
| id | doaj.art-e3859324d79b4c1584f91b10f12153fd |
| institution | Directory Open Access Journal |
| issn | 2048-7177 |
| language | English |
| last_indexed | 2024-03-11T19:00:53Z |
| publishDate | 2023-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food Science & Nutrition |
| spelling | doaj.art-e3859324d79b4c1584f91b10f12153fd2023-10-10T12:30:21ZengWileyFood Science & Nutrition2048-71772023-10-0111106106611510.1002/fsn3.3547The effect of anthocyanin from Dioscorea alata L. after purification, identification on antioxidant capacity in micePingfei Qiu0Junpu Chen1Junlong Wu2Qin Wang3Yanrong Hu4Xiaochun Li5Huiyu Shi6Xuemei Wang7Animal Nutrition, Reproduction and Breeding Laboratory, School of Animal Science and Technology Hainan University Haikou ChinaAnimal Nutrition, Reproduction and Breeding Laboratory, School of Animal Science and Technology Hainan University Haikou ChinaAnimal Nutrition, Reproduction and Breeding Laboratory, School of Animal Science and Technology Hainan University Haikou ChinaAnimal Nutrition, Reproduction and Breeding Laboratory, School of Animal Science and Technology Hainan University Haikou ChinaAnimal Nutrition, Reproduction and Breeding Laboratory, School of Animal Science and Technology Hainan University Haikou ChinaAnimal Nutrition, Reproduction and Breeding Laboratory, School of Animal Science and Technology Hainan University Haikou ChinaAnimal Nutrition, Reproduction and Breeding Laboratory, School of Animal Science and Technology Hainan University Haikou ChinaAnimal Nutrition, Reproduction and Breeding Laboratory, School of Animal Science and Technology Hainan University Haikou ChinaAbstract Increasing findings devote to searching for natural active compositions as additives to ameliorate health status. Anthocyanin, water‐soluble natural pigment, has been concerned due to its favorable antioxidant activity. In this study, we purified anthocyanin from Dioscorea alata L., identified its compounds, and evaluated its antioxidant properties. The results indicated that the purity of anthocyanin increased to 39.59 ± 1.56%, 60.18 ± 1.97%, and 81.08 ± 1.97% after purification via AB‐8 macroporous resin, Sep‐Pak C18 solid phase, and LH‐20 Sephadex stepwise. Ultra‐performance liquid chromatography tandem mass spectrometer results indicated that paeoniflorin‐3,5‐O‐dihexoside, petunin‐3‐O‐feruloyl‐glucoside‐5‐O‐glucoside, cyanidin‐3‐O‐feruloyl glucoside‐5‐O‐glucoside, cyanidin‐3‐O‐sophoroside, and petunin‐3,5‐O‐dihexoside were the major compounds. The purified anthocyanin exhibited stronger antioxidant activity than the unpurified extract and ascorbic acid, whereas weaker than that of cyanidin‐3‐O‐glucoside in general, which was assessed using DPPH, ABTS, and Fe3+ reducing capacity methods. Moreover, the purified anthocyanin increased GSH‐Px, total antioxidant capacity, and superoxide dismutase activities and decreased malondialdehyde concentration on serum in mice after administering lipopolysaccharide for 24 h (p < .05). To summarize, the purified anthocyanin boasts more outstanding antioxidant properties than that of crude extracts. These results provide a reference with source of anthocyanin and it is conducive to use Dioscorea alata L. resources.https://doi.org/10.1002/fsn3.3547anthocyaninantioxidant capacityDioscorea alata L.macroporous |
| spellingShingle | Pingfei Qiu Junpu Chen Junlong Wu Qin Wang Yanrong Hu Xiaochun Li Huiyu Shi Xuemei Wang The effect of anthocyanin from Dioscorea alata L. after purification, identification on antioxidant capacity in mice Food Science & Nutrition anthocyanin antioxidant capacity Dioscorea alata L. macroporous |
| title | The effect of anthocyanin from Dioscorea alata L. after purification, identification on antioxidant capacity in mice |
| title_full | The effect of anthocyanin from Dioscorea alata L. after purification, identification on antioxidant capacity in mice |
| title_fullStr | The effect of anthocyanin from Dioscorea alata L. after purification, identification on antioxidant capacity in mice |
| title_full_unstemmed | The effect of anthocyanin from Dioscorea alata L. after purification, identification on antioxidant capacity in mice |
| title_short | The effect of anthocyanin from Dioscorea alata L. after purification, identification on antioxidant capacity in mice |
| title_sort | effect of anthocyanin from dioscorea alata l after purification identification on antioxidant capacity in mice |
| topic | anthocyanin antioxidant capacity Dioscorea alata L. macroporous |
| url | https://doi.org/10.1002/fsn3.3547 |
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