Rapid, simple, and effective strategy to produce monoclonal antibodies targeting protein structures using hybridoma technology

Rapid, simple, and effective strategy to produce monoclonal antibodies targeting protein structures using hybridoma technology

Highlights ・MIHS-SAST screening generates mAbs that target protein structures. ・One-fourth of the mAbs recognized stereospecific epitopes of the protein. ・Two fluorometric parameters aided in selecting antibodies with strong binding constants.

Bibliographic Details
Main Authors: Atsumi Sakaguchi, Yoichiro Tanaka, Eiki Shoji, Teppei Takeshima, Rina Sakamaki, Takao Matsuba, Yasuyuki Kurihara
Format: Article
Language:English
Published: BMC 2023-03-01
Series:Journal of Biological Engineering
Subjects:
Monoclonal antibody
Flow cytometer
Conformation-specific antibody
ELISA
Hybridoma technology
Online Access:https://doi.org/10.1186/s13036-023-00345-9

Internet

https://doi.org/10.1186/s13036-023-00345-9

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