Optimization of real-time PCR protocols from lymph node bovine tissue for direct detection of Mycobacterium tuberculosis complex

Optimization of real-time PCR protocols from lymph node bovine tissue for direct detection of Mycobacterium tuberculosis complex

ABSTRACT Bovine tuberculosis (bTB) is a zoonotic disease and a global health problem that is subjected to obligatory eradication programs in the European Union. Microbiological culture is an imperfect technique for bTB diagnosis. This study aims to compare and validate two DNA isolation protocols an...

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Bibliographic Details
Main Authors: Eduardo Vera-Salmoral, Jaime Gómez-Laguna, Ángela Galán-Relaño, Inés Ruedas-Torres, Librado Carrasco, Inmaculada Luque, Belén Huerta, José María Sánchez-Carvajal
Format: Article
Language:English
Published: American Society for Microbiology 2023-10-01
Series:Microbiology Spectrum
Subjects:
direct real-time PCR
extraction protocol
fresh tissue
IS6110
IS4
mpb70
Online Access:https://journals.asm.org/doi/10.1128/spectrum.00348-23

Internet

https://journals.asm.org/doi/10.1128/spectrum.00348-23

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