Higher resolution protein band visualisation via improvement of colloidal CBB-G staining by gel fixation

Higher resolution protein band visualisation via improvement of colloidal CBB-G staining by gel fixation

Abstract Background Gel staining is a crucial step that allows the visualisation of proteins separated through SDS-PAGE. Colloidal Coomassie Brilliant Blue-G (CBB-G) staining is among the commonly used visualisation methods due to several factors such as compatibility with mass spectrometry (MS) ana...

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Bibliographic Details
Main Authors: Nikson Fatt-Ming Chong, Hasnain Hussain, Nur Ezzati Hamdin, David Hong-Sheng Wee, Mehvish Nisar, Wei-Jie Yan, Benjamin Yii Chung Lau, Norasfaliza Rahmad
Format: Article
Language:English
Published: SpringerOpen 2022-05-01
Series:Chemical and Biological Technologies in Agriculture
Subjects:
SDS-PAGE
Protein staining
Colloidal
Coomassie
Gel fixation
Proteomics
Online Access:https://doi.org/10.1186/s40538-022-00297-0

Internet

https://doi.org/10.1186/s40538-022-00297-0

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