Profiling the peripheral blood T cell receptor repertoires of gastric cancer patients
Cancer driven by somatic mutations may express neoantigens that can trigger T-cell immune responses. Since T-cell receptor (TCR) repertoires play critical roles in anti-tumor immune responses for oncology, next-generation sequencing (NGS) was used to profile the hypervariable complementarity-determi...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-07-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.848113/full |
| _version_ | 1818161643733909504 |
|---|---|
| author | Mengyao Wang Peng Gao Laifeng Ren Jingjing Duan Silu Yang Haina Wang Hongxia Wang Junning Sun Xiaoyan Gao Bo Li Shuaicheng Li Wen Su |
| author_facet | Mengyao Wang Peng Gao Laifeng Ren Jingjing Duan Silu Yang Haina Wang Hongxia Wang Junning Sun Xiaoyan Gao Bo Li Shuaicheng Li Wen Su |
| author_sort | Mengyao Wang |
| collection | DOAJ |
| description | Cancer driven by somatic mutations may express neoantigens that can trigger T-cell immune responses. Since T-cell receptor (TCR) repertoires play critical roles in anti-tumor immune responses for oncology, next-generation sequencing (NGS) was used to profile the hypervariable complementarity-determining region 3 (CDR3) of the TCR-beta chain in peripheral blood samples from 68 gastric cancer patients and 49 healthy controls. We found that most hyper-expanded CDR3 are individual-specific, and the gene usage of TRBV3-1 is more frequent in the tumor group regardless of tumor stage than in the healthy control group. We identified 374 hyper-expanded tumor-specific CDR3, which may play a vital role in anti-tumor immune responses. The patients with stage IV gastric cancer have higher EBV-specific CDR3 abundance than the control. In conclusion, analysis of the peripheral blood TCR repertoires may provide the biomarker for gastric cancer prognosis and guide future immunotherapy. |
| first_indexed | 2024-12-11T16:21:02Z |
| format | Article |
| id | doaj.art-e3d864afb0b44907b7f5257937413f8d |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-11T16:21:02Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-e3d864afb0b44907b7f5257937413f8d2022-12-22T00:58:51ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-07-011310.3389/fimmu.2022.848113848113Profiling the peripheral blood T cell receptor repertoires of gastric cancer patientsMengyao Wang0Peng Gao1Laifeng Ren2Jingjing Duan3Silu Yang4Haina Wang5Hongxia Wang6Junning Sun7Xiaoyan Gao8Bo Li9Shuaicheng Li10Wen Su11Department of Computer Science, City University of Hong Kong Shenzhen Research Institute, Shenzhen, ChinaBGI-Shenzhen, Shenzhen, ChinaDepartment of Immunology, Shanxi Province Cancer Hospital/ Shanxi Hospital Affiliated to Cancer Hospital, Chinese Academy of Medical Sciences/Cancer Hospital Affiliated to Shanxi Medical University, Taiyuan, ChinaDepartment of Immunology, Shanxi Province Cancer Hospital/ Shanxi Hospital Affiliated to Cancer Hospital, Chinese Academy of Medical Sciences/Cancer Hospital Affiliated to Shanxi Medical University, Taiyuan, ChinaDepartment of Immunology, Shanxi Province Cancer Hospital/ Shanxi Hospital Affiliated to Cancer Hospital, Chinese Academy of Medical Sciences/Cancer Hospital Affiliated to Shanxi Medical University, Taiyuan, ChinaDepartment of Immunology, Shanxi Province Cancer Hospital/ Shanxi Hospital Affiliated to Cancer Hospital, Chinese Academy of Medical Sciences/Cancer Hospital Affiliated to Shanxi Medical University, Taiyuan, ChinaDepartment of Immunology, Shanxi Province Cancer Hospital/ Shanxi Hospital Affiliated to Cancer Hospital, Chinese Academy of Medical Sciences/Cancer Hospital Affiliated to Shanxi Medical University, Taiyuan, ChinaDepartment of Immunology, Shanxi Province Cancer Hospital/ Shanxi Hospital Affiliated to Cancer Hospital, Chinese Academy of Medical Sciences/Cancer Hospital Affiliated to Shanxi Medical University, Taiyuan, ChinaBGI-Shenzhen, Shenzhen, ChinaBGI-Shenzhen, Shenzhen, ChinaDepartment of Computer Science, City University of Hong Kong Shenzhen Research Institute, Shenzhen, ChinaDepartment of Immunology, Shanxi Province Cancer Hospital/ Shanxi Hospital Affiliated to Cancer Hospital, Chinese Academy of Medical Sciences/Cancer Hospital Affiliated to Shanxi Medical University, Taiyuan, ChinaCancer driven by somatic mutations may express neoantigens that can trigger T-cell immune responses. Since T-cell receptor (TCR) repertoires play critical roles in anti-tumor immune responses for oncology, next-generation sequencing (NGS) was used to profile the hypervariable complementarity-determining region 3 (CDR3) of the TCR-beta chain in peripheral blood samples from 68 gastric cancer patients and 49 healthy controls. We found that most hyper-expanded CDR3 are individual-specific, and the gene usage of TRBV3-1 is more frequent in the tumor group regardless of tumor stage than in the healthy control group. We identified 374 hyper-expanded tumor-specific CDR3, which may play a vital role in anti-tumor immune responses. The patients with stage IV gastric cancer have higher EBV-specific CDR3 abundance than the control. In conclusion, analysis of the peripheral blood TCR repertoires may provide the biomarker for gastric cancer prognosis and guide future immunotherapy.https://www.frontiersin.org/articles/10.3389/fimmu.2022.848113/fullTCR repertoirenext-generation sequencinggastric cancerbiomarkerantitumor immunity |
| spellingShingle | Mengyao Wang Peng Gao Laifeng Ren Jingjing Duan Silu Yang Haina Wang Hongxia Wang Junning Sun Xiaoyan Gao Bo Li Shuaicheng Li Wen Su Profiling the peripheral blood T cell receptor repertoires of gastric cancer patients Frontiers in Immunology TCR repertoire next-generation sequencing gastric cancer biomarker antitumor immunity |
| title | Profiling the peripheral blood T cell receptor repertoires of gastric cancer patients |
| title_full | Profiling the peripheral blood T cell receptor repertoires of gastric cancer patients |
| title_fullStr | Profiling the peripheral blood T cell receptor repertoires of gastric cancer patients |
| title_full_unstemmed | Profiling the peripheral blood T cell receptor repertoires of gastric cancer patients |
| title_short | Profiling the peripheral blood T cell receptor repertoires of gastric cancer patients |
| title_sort | profiling the peripheral blood t cell receptor repertoires of gastric cancer patients |
| topic | TCR repertoire next-generation sequencing gastric cancer biomarker antitumor immunity |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.848113/full |
| work_keys_str_mv | AT mengyaowang profilingtheperipheralbloodtcellreceptorrepertoiresofgastriccancerpatients AT penggao profilingtheperipheralbloodtcellreceptorrepertoiresofgastriccancerpatients AT laifengren profilingtheperipheralbloodtcellreceptorrepertoiresofgastriccancerpatients AT jingjingduan profilingtheperipheralbloodtcellreceptorrepertoiresofgastriccancerpatients AT siluyang profilingtheperipheralbloodtcellreceptorrepertoiresofgastriccancerpatients AT hainawang profilingtheperipheralbloodtcellreceptorrepertoiresofgastriccancerpatients AT hongxiawang profilingtheperipheralbloodtcellreceptorrepertoiresofgastriccancerpatients AT junningsun profilingtheperipheralbloodtcellreceptorrepertoiresofgastriccancerpatients AT xiaoyangao profilingtheperipheralbloodtcellreceptorrepertoiresofgastriccancerpatients AT boli profilingtheperipheralbloodtcellreceptorrepertoiresofgastriccancerpatients AT shuaichengli profilingtheperipheralbloodtcellreceptorrepertoiresofgastriccancerpatients AT wensu profilingtheperipheralbloodtcellreceptorrepertoiresofgastriccancerpatients |