Evaluation of Rapid Extraction Methods Coupled with a Recombinase Polymerase Amplification Assay for Point-of-Need Diagnosis of Post-Kala-Azar Dermal Leishmaniasis

Evaluation of Rapid Extraction Methods Coupled with a Recombinase Polymerase Amplification Assay for Point-of-Need Diagnosis of Post-Kala-Azar Dermal Leishmaniasis

To detect Post-kala-azar leishmaniasis (PKDL) cases, several molecular methods with promising diagnostic efficacy have been developed that involve complicated and expensive DNA extraction methods, thus limiting their application in resource-poor settings. As an alternative, we evaluated two rapid DN...

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Bibliographic Details
Main Authors: Rajashree Chowdhury, Prakash Ghosh, Md. Anik Ashfaq Khan, Faria Hossain, Khaledul Faisal, Rupen Nath, James Baker, Ahmed Abd El Wahed, Shomik Maruf, Proggananda Nath, Debashis Ghosh, Md. Masud-Ur-Rashid, Md. Utba Bin Rashid, Malcolm S. Duthie, Dinesh Mondal
Format: Article
Language:English
Published: MDPI AG 2020-06-01
Series:Tropical Medicine and Infectious Disease
Subjects:
post-kala-azar dermal leishmaniasis (PKDL)
point-of-need diagnosis
DNA extraction
recombinase polymerase amplification (RPA)
real-time PCR
Online Access:https://www.mdpi.com/2414-6366/5/2/95

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https://www.mdpi.com/2414-6366/5/2/95

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