Selection and Validation of Reference Genes for RT-qPCR Analysis of Gene Expression in <i>Nicotiana benthamiana</i> upon Single Infections by 11 Positive-Sense Single-Stranded RNA Viruses from Four Genera

Selection and Validation of Reference Genes for RT-qPCR Analysis of Gene Expression in <i>Nicotiana benthamiana</i> upon Single Infections by 11 Positive-Sense Single-Stranded RNA Viruses from Four Genera

Quantitative real-time PCR (RT-qPCR) is a widely used method for studying alterations in gene expression upon infections caused by diverse pathogens such as viruses. Positive-sense single-stranded (ss(+)) RNA viruses form a major part of all known plant viruses, and some of them are damaging pathoge...

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Bibliographic Details
Main Authors: Ge Zhang, Zhuo Zhang, Qionglian Wan, Huijie Zhou, Mengting Jiao, Hongying Zheng, Yuwen Lu, Shaofei Rao, Guanwei Wu, Jianping Chen, Fei Yan, Jiejun Peng, Jian Wu
Format: Article
Language:English
Published: MDPI AG 2023-02-01
Series:Plants
Subjects:
RT-qPCR
reference gene
positive-sense single-stranded RNA
<i>Nicotiana benthamiana</i>
Online Access:https://www.mdpi.com/2223-7747/12/4/857

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https://www.mdpi.com/2223-7747/12/4/857

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