Protocol to detect in vitro and in cell ubiquitylation of mitochondrial DNA polymerase gamma by mitochondrial E3 ligase MITOL

Protocol to detect in vitro and in cell ubiquitylation of mitochondrial DNA polymerase gamma by mitochondrial E3 ligase MITOL

Summary: Mitochondrial polymerase gamma (PolγA) is the only replicative polymerase in mitochondria. To determine PolγA ubiquitylation in cells, Flag-PolγA and MITOL are overexpressed, and subsequently the immunoprecipitated Flag-PolγA is checked for ubiquitylation. Alternately, in vitro synthesized...

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Bibliographic Details
Main Authors: Mansoor Hussain, Shabnam Saifi, Aftab Mohammed, Sagar Sengupta
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Cell biology
Genetics
Molecular biology
Protein biochemistry
Protein expression and purification
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722005901

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http://www.sciencedirect.com/science/article/pii/S2666166722005901

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