Antiphospholipid antibodies induce proinflammatory and procoagulant pathways in endothelial cells
Antiphospholipid syndrome (APS) is an autoimmune thrombophilia characterized by recurrent thrombotic events and/or pregnancy morbidity in the presence of antiphospholipid antibodies detected either as anti-cardiolipin, anti-β2 Glycoprotein I (anti-β2GPI) or Lupus anticoagulant (LA). Endothelial dere...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Journal of Translational Autoimmunity |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589909023000151 |
| _version_ | 1797801009457659904 |
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| author | Markos Patsouras Eirini Alexopoulou Spyros Foutadakis Eirini Tsiki Panagiota Karagianni Marios Agelopoulos Panayiotis G. Vlachoyiannopoulos |
| author_facet | Markos Patsouras Eirini Alexopoulou Spyros Foutadakis Eirini Tsiki Panagiota Karagianni Marios Agelopoulos Panayiotis G. Vlachoyiannopoulos |
| author_sort | Markos Patsouras |
| collection | DOAJ |
| description | Antiphospholipid syndrome (APS) is an autoimmune thrombophilia characterized by recurrent thrombotic events and/or pregnancy morbidity in the presence of antiphospholipid antibodies detected either as anti-cardiolipin, anti-β2 Glycoprotein I (anti-β2GPI) or Lupus anticoagulant (LA). Endothelial deregulation characterizes the syndrome. To address gene expression changes accompanying the development of autoimmune phenotype in endothelial cells in the context of APS, we performed transcriptomics analysis in Human Umbilical Vein Endothelial Cells (HUVECs) stimulated with IgG from APS patients and β2GPI, followed by intersection of RNA-seq data with published microarray and ChIP-seq results (Chromatin Immunoprecipitation). Our strategy revealed that during HUVEC activation diverse signaling pathways such as TNF-α, TGF-β, MAPK38, and Hippo are triggered as indicated by Gene Ontology (GO) classification and pathway analysis. Finally, cell biology approaches performed side-by-side in naïve and stimulated cultured HUVECs, as well as, in placenta specimens derived from Healthy donors (HDs) and APS-patients verified the evolution of an APS-characteristic gene expression program in endothelial cells during the initial stages of the disease's development. |
| first_indexed | 2024-03-13T04:43:54Z |
| format | Article |
| id | doaj.art-e42b1d353c8f4335bd63426067af4208 |
| institution | Directory Open Access Journal |
| issn | 2589-9090 |
| language | English |
| last_indexed | 2024-03-13T04:43:54Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Translational Autoimmunity |
| spelling | doaj.art-e42b1d353c8f4335bd63426067af42082023-06-19T04:29:19ZengElsevierJournal of Translational Autoimmunity2589-90902023-01-016100202Antiphospholipid antibodies induce proinflammatory and procoagulant pathways in endothelial cellsMarkos Patsouras0Eirini Alexopoulou1Spyros Foutadakis2Eirini Tsiki3Panagiota Karagianni4Marios Agelopoulos5Panayiotis G. Vlachoyiannopoulos6Department of Pathophysiology, Medical School, National and Kapodistrian University of Athens, GreeceDepartment of Pathophysiology, Medical School, National and Kapodistrian University of Athens, GreeceCenter of Basic Research, Biomedical Research Foundation, Academy of Athens, 4 Soranou Ephessiou Street, Athens, 11527, GreeceDepartment of Pathophysiology, Medical School, National and Kapodistrian University of Athens, GreeceDepartment of Pathophysiology, Medical School, National and Kapodistrian University of Athens, GreeceCenter of Basic Research, Biomedical Research Foundation, Academy of Athens, 4 Soranou Ephessiou Street, Athens, 11527, GreeceDepartment of Pathophysiology, Medical School, National and Kapodistrian University of Athens, Greece; Corresponding author. Department of Pathophysiology, Medical School, National and Kapodistrian University of Athens, 75 Mikras Asias str, 11527, Athens, Greece.Antiphospholipid syndrome (APS) is an autoimmune thrombophilia characterized by recurrent thrombotic events and/or pregnancy morbidity in the presence of antiphospholipid antibodies detected either as anti-cardiolipin, anti-β2 Glycoprotein I (anti-β2GPI) or Lupus anticoagulant (LA). Endothelial deregulation characterizes the syndrome. To address gene expression changes accompanying the development of autoimmune phenotype in endothelial cells in the context of APS, we performed transcriptomics analysis in Human Umbilical Vein Endothelial Cells (HUVECs) stimulated with IgG from APS patients and β2GPI, followed by intersection of RNA-seq data with published microarray and ChIP-seq results (Chromatin Immunoprecipitation). Our strategy revealed that during HUVEC activation diverse signaling pathways such as TNF-α, TGF-β, MAPK38, and Hippo are triggered as indicated by Gene Ontology (GO) classification and pathway analysis. Finally, cell biology approaches performed side-by-side in naïve and stimulated cultured HUVECs, as well as, in placenta specimens derived from Healthy donors (HDs) and APS-patients verified the evolution of an APS-characteristic gene expression program in endothelial cells during the initial stages of the disease's development.http://www.sciencedirect.com/science/article/pii/S2589909023000151Antiphospholipid syndrome (APS)Transcriptomics analysisComputational biology toolsInflammatory and procoagulant phenotypeTranscriptional regulatorsGene expression programs |
| spellingShingle | Markos Patsouras Eirini Alexopoulou Spyros Foutadakis Eirini Tsiki Panagiota Karagianni Marios Agelopoulos Panayiotis G. Vlachoyiannopoulos Antiphospholipid antibodies induce proinflammatory and procoagulant pathways in endothelial cells Journal of Translational Autoimmunity Antiphospholipid syndrome (APS) Transcriptomics analysis Computational biology tools Inflammatory and procoagulant phenotype Transcriptional regulators Gene expression programs |
| title | Antiphospholipid antibodies induce proinflammatory and procoagulant pathways in endothelial cells |
| title_full | Antiphospholipid antibodies induce proinflammatory and procoagulant pathways in endothelial cells |
| title_fullStr | Antiphospholipid antibodies induce proinflammatory and procoagulant pathways in endothelial cells |
| title_full_unstemmed | Antiphospholipid antibodies induce proinflammatory and procoagulant pathways in endothelial cells |
| title_short | Antiphospholipid antibodies induce proinflammatory and procoagulant pathways in endothelial cells |
| title_sort | antiphospholipid antibodies induce proinflammatory and procoagulant pathways in endothelial cells |
| topic | Antiphospholipid syndrome (APS) Transcriptomics analysis Computational biology tools Inflammatory and procoagulant phenotype Transcriptional regulators Gene expression programs |
| url | http://www.sciencedirect.com/science/article/pii/S2589909023000151 |
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