A Yeast BiFC-seq Method for Genome-wide Interactome Mapping

A Yeast BiFC-seq Method for Genome-wide Interactome Mapping

Genome-wide physical protein–protein interaction (PPI) mapping remains a major challenge for current technologies. Here, we reported a high-efficiency BiFC-seq method, yeast-enhanced green fluorescent protein-based bimolecular fluorescence complementation (yEGFP-BiFC) coupled with next-generation DN...

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Bibliographic Details
Main Authors: Limin Shang, Yuehui Zhang, Yuchen Liu, Chaozhi Jin, Yanzhi Yuan, Chunyan Tian, Ming Ni, Xiaochen Bo, Li Zhang, Dong Li, Fuchu He, Jian Wang
Format: Article
Language:English
Published: Elsevier 2022-08-01
Series:Genomics, Proteomics & Bioinformatics
Subjects:
Bimolecular fluorescence complementation
Protein–protein interaction
High-throughput
Next-generation sequencing
Online Access:http://www.sciencedirect.com/science/article/pii/S1672022921001595

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http://www.sciencedirect.com/science/article/pii/S1672022921001595

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