Determination of guava (Psidium guajava L.) leaf phenolic compounds using HPLC-DAD-QTOF-MS
Markets of different countries have proposed guava tea infusions as a drink that can modulate the glycaemic index in blood. This property has been attributed to the phenolic compounds contained in guava leaves. However, phenolic profile of guava leaves is still not well-known. Based on this informat...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-04-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464616000505 |
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| author | Elixabet Díaz-de-Cerio Ana María Gómez-Caravaca Vito Verardo Alberto Fernández-Gutiérrez Antonio Segura-Carretero |
| author_facet | Elixabet Díaz-de-Cerio Ana María Gómez-Caravaca Vito Verardo Alberto Fernández-Gutiérrez Antonio Segura-Carretero |
| author_sort | Elixabet Díaz-de-Cerio |
| collection | DOAJ |
| description | Markets of different countries have proposed guava tea infusions as a drink that can modulate the glycaemic index in blood. This property has been attributed to the phenolic compounds contained in guava leaves. However, phenolic profile of guava leaves is still not well-known. Based on this information, different ethanol/water mixtures were used to extract the phenolic compounds in guava leaves. Phenolic identification was carried out by HPLC-ESI-QTOF-MS in guava leaves from pomifera and pyrifera varieties; moreover, the antioxidant activities of the ethanolic extracts were determined by TEAC and FRAP methods. To sum up, seventy-two phenolic compounds were identified. To our knowledge, twelve of them were determined for the first time in guava leaves. The highest amount of phenolic compounds was found in EtOH/H2O 80:20 (v/v) mixture. Furthermore, pyrifera var. showed higher concentration of phenolic compounds than pomifera var. (113.34 vs. 86.12 mg/g leaf d.w.) and also greater antioxidant capacity. |
| first_indexed | 2024-12-14T10:07:49Z |
| format | Article |
| id | doaj.art-e44e7b0f5ec546f0a612090090162496 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-14T10:07:49Z |
| publishDate | 2016-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-e44e7b0f5ec546f0a6120900901624962022-12-21T23:07:05ZengElsevierJournal of Functional Foods1756-46462016-04-0122376388Determination of guava (Psidium guajava L.) leaf phenolic compounds using HPLC-DAD-QTOF-MSElixabet Díaz-de-Cerio0Ana María Gómez-Caravaca1Vito Verardo2Alberto Fernández-Gutiérrez3Antonio Segura-Carretero4Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avd. Fuentenueva s/n, 18071, Granada, Spain; Functional Food Research and Development Center, Health Science Technological Park, Avd. del Conocimiento, Bioregion building, 18100, Granada, SpainDepartment of Analytical Chemistry, Faculty of Sciences, University of Granada, Avd. Fuentenueva s/n, 18071, Granada, Spain; Functional Food Research and Development Center, Health Science Technological Park, Avd. del Conocimiento, Bioregion building, 18100, Granada, SpainDepartment of Chemistry and Physics (Analytical Chemistry Area) and Research Centre for Agricultural and Food Biotechnology (BITAL), Agrifood Campus of International Excellence (ceiA3), University of Almería, Carretera de Sacramento s/n, E-04120 Almería, Spain; Corresponding author. Department of Chemistry and Physics (Analytical Chemistry Area) and Research Centre for Agricultural and Food Biotechnology (BITAL), Agrifood Campus of International Excellence (ceiA3), University of Almería, Carretera de Sacramento s/n, E-04120 Almería, Spain. Tel.: +34 958 637 206; fax: +34 958 637 083.Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avd. Fuentenueva s/n, 18071, Granada, Spain; Functional Food Research and Development Center, Health Science Technological Park, Avd. del Conocimiento, Bioregion building, 18100, Granada, SpainDepartment of Analytical Chemistry, Faculty of Sciences, University of Granada, Avd. Fuentenueva s/n, 18071, Granada, Spain; Functional Food Research and Development Center, Health Science Technological Park, Avd. del Conocimiento, Bioregion building, 18100, Granada, SpainMarkets of different countries have proposed guava tea infusions as a drink that can modulate the glycaemic index in blood. This property has been attributed to the phenolic compounds contained in guava leaves. However, phenolic profile of guava leaves is still not well-known. Based on this information, different ethanol/water mixtures were used to extract the phenolic compounds in guava leaves. Phenolic identification was carried out by HPLC-ESI-QTOF-MS in guava leaves from pomifera and pyrifera varieties; moreover, the antioxidant activities of the ethanolic extracts were determined by TEAC and FRAP methods. To sum up, seventy-two phenolic compounds were identified. To our knowledge, twelve of them were determined for the first time in guava leaves. The highest amount of phenolic compounds was found in EtOH/H2O 80:20 (v/v) mixture. Furthermore, pyrifera var. showed higher concentration of phenolic compounds than pomifera var. (113.34 vs. 86.12 mg/g leaf d.w.) and also greater antioxidant capacity.http://www.sciencedirect.com/science/article/pii/S1756464616000505Guava (Psidium guajava L.) leavesFlavan-3-olsFlavonolsGallic and ellagic derivativesPhenolic compoundsHPLC-DAD-QTOF-MS |
| spellingShingle | Elixabet Díaz-de-Cerio Ana María Gómez-Caravaca Vito Verardo Alberto Fernández-Gutiérrez Antonio Segura-Carretero Determination of guava (Psidium guajava L.) leaf phenolic compounds using HPLC-DAD-QTOF-MS Journal of Functional Foods Guava (Psidium guajava L.) leaves Flavan-3-ols Flavonols Gallic and ellagic derivatives Phenolic compounds HPLC-DAD-QTOF-MS |
| title | Determination of guava (Psidium guajava L.) leaf phenolic compounds using HPLC-DAD-QTOF-MS |
| title_full | Determination of guava (Psidium guajava L.) leaf phenolic compounds using HPLC-DAD-QTOF-MS |
| title_fullStr | Determination of guava (Psidium guajava L.) leaf phenolic compounds using HPLC-DAD-QTOF-MS |
| title_full_unstemmed | Determination of guava (Psidium guajava L.) leaf phenolic compounds using HPLC-DAD-QTOF-MS |
| title_short | Determination of guava (Psidium guajava L.) leaf phenolic compounds using HPLC-DAD-QTOF-MS |
| title_sort | determination of guava psidium guajava l leaf phenolic compounds using hplc dad qtof ms |
| topic | Guava (Psidium guajava L.) leaves Flavan-3-ols Flavonols Gallic and ellagic derivatives Phenolic compounds HPLC-DAD-QTOF-MS |
| url | http://www.sciencedirect.com/science/article/pii/S1756464616000505 |
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