Pancreatic islet cell type–specific transcriptomic changes during pregnancy and postpartum
Summary: Pancreatic β-cell mass expands during pregnancy and regresses in the postpartum period in conjunction with dynamic metabolic demands on maternal glucose homeostasis. To understand transcriptional changes driving these adaptations in β-cells and other islet cell types, we performed single-ce...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-04-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223005163 |
| _version_ | 1797854498803154944 |
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| author | Jin-Yong Chung Yongjie Ma Dingguo Zhang Hayden H. Bickerton Eric Stokes Sweta B. Patel Hubert M. Tse Joseph Feduska Rob S. Welner Ronadip R. Banerjee |
| author_facet | Jin-Yong Chung Yongjie Ma Dingguo Zhang Hayden H. Bickerton Eric Stokes Sweta B. Patel Hubert M. Tse Joseph Feduska Rob S. Welner Ronadip R. Banerjee |
| author_sort | Jin-Yong Chung |
| collection | DOAJ |
| description | Summary: Pancreatic β-cell mass expands during pregnancy and regresses in the postpartum period in conjunction with dynamic metabolic demands on maternal glucose homeostasis. To understand transcriptional changes driving these adaptations in β-cells and other islet cell types, we performed single-cell RNA sequencing on islets from virgin, late gestation, and early postpartum mice. We identified transcriptional signatures unique to gestation and the postpartum in β-cells, including induction of the AP-1 transcription factor subunits and other genes involved in the immediate-early response (IEGs). In addition, we found pregnancy and postpartum-induced changes differed within each endocrine cell type, and in endothelial cells and antigen-presenting cells within islets. Together, our data reveal insights into cell type–specific transcriptional changes responsible for adaptations by islet cells to pregnancy and their resolution postpartum. |
| first_indexed | 2024-04-09T20:08:08Z |
| format | Article |
| id | doaj.art-e462455c24ea41eba113a99958e8fbf9 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-09T20:08:08Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-e462455c24ea41eba113a99958e8fbf92023-04-01T08:51:08ZengElsevieriScience2589-00422023-04-01264106439Pancreatic islet cell type–specific transcriptomic changes during pregnancy and postpartumJin-Yong Chung0Yongjie Ma1Dingguo Zhang2Hayden H. Bickerton3Eric Stokes4Sweta B. Patel5Hubert M. Tse6Joseph Feduska7Rob S. Welner8Ronadip R. Banerjee9Division of Endocrinology, Diabetes & Metabolism, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21224, USADepartment of Pharmacology, the University of Alabama at Birmingham School of Medicine, Birmingham, AL 35294, USADivision of Endocrinology, Diabetes and Metabolism, Department of Medicine, The University of Alabama at Birmingham School of Medicine, Birmingham, AL 35294, USADivision of Endocrinology, Diabetes and Metabolism, Department of Medicine, The University of Alabama at Birmingham School of Medicine, Birmingham, AL 35294, USADepartment of Pharmacology, University of Colorado Denver/Anschutz, Aurora, CO 80045, USADivision of Hematology and Oncology, Department of Medicine, The University of Alabama at Birmingham School of Medicine, Birmingham, AL 35294, USADepartment of Microbiology, the University of Alabama at Birmingham School of Medicine, Birmingham, AL 35294, USADepartment of Microbiology, the University of Alabama at Birmingham School of Medicine, Birmingham, AL 35294, USADivision of Hematology and Oncology, Department of Medicine, The University of Alabama at Birmingham School of Medicine, Birmingham, AL 35294, USADivision of Endocrinology, Diabetes & Metabolism, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21224, USA; Corresponding authorSummary: Pancreatic β-cell mass expands during pregnancy and regresses in the postpartum period in conjunction with dynamic metabolic demands on maternal glucose homeostasis. To understand transcriptional changes driving these adaptations in β-cells and other islet cell types, we performed single-cell RNA sequencing on islets from virgin, late gestation, and early postpartum mice. We identified transcriptional signatures unique to gestation and the postpartum in β-cells, including induction of the AP-1 transcription factor subunits and other genes involved in the immediate-early response (IEGs). In addition, we found pregnancy and postpartum-induced changes differed within each endocrine cell type, and in endothelial cells and antigen-presenting cells within islets. Together, our data reveal insights into cell type–specific transcriptional changes responsible for adaptations by islet cells to pregnancy and their resolution postpartum.http://www.sciencedirect.com/science/article/pii/S2589004223005163PregnancyMolecular physiologyTranscriptomics |
| spellingShingle | Jin-Yong Chung Yongjie Ma Dingguo Zhang Hayden H. Bickerton Eric Stokes Sweta B. Patel Hubert M. Tse Joseph Feduska Rob S. Welner Ronadip R. Banerjee Pancreatic islet cell type–specific transcriptomic changes during pregnancy and postpartum iScience Pregnancy Molecular physiology Transcriptomics |
| title | Pancreatic islet cell type–specific transcriptomic changes during pregnancy and postpartum |
| title_full | Pancreatic islet cell type–specific transcriptomic changes during pregnancy and postpartum |
| title_fullStr | Pancreatic islet cell type–specific transcriptomic changes during pregnancy and postpartum |
| title_full_unstemmed | Pancreatic islet cell type–specific transcriptomic changes during pregnancy and postpartum |
| title_short | Pancreatic islet cell type–specific transcriptomic changes during pregnancy and postpartum |
| title_sort | pancreatic islet cell type specific transcriptomic changes during pregnancy and postpartum |
| topic | Pregnancy Molecular physiology Transcriptomics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223005163 |
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