Peroxiredoxin1, a novel regulator of pronephros development, influences retinoic acid and Wnt signaling by controlling ROS levels
Abstract Peroxiredoxin1 (Prdx1) is an antioxidant enzyme belonging to the peroxiredoxin family of proteins. Prdx1 catalyzes the reduction of H2O2 and alkyl hydroperoxide and plays an important role in different biological processes. Prdx1 also participates in various age-related diseases and cancers...
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2017-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-09262-6 |
| _version_ | 1818842432519798784 |
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| author | Soomin Chae Hyun-Kyung Lee Yoo-Kyung Kim Hyo Jung Sim Yoorim Ji Chowon Kim Tayaba Ismail Jeen-Woo Park Oh-Shin Kwon Beom-Sik Kang Dong-Seok Lee Jong-Sup Bae Sang-Hyun Kim Kyoung-Jin Min Taeg Kyu Kwon Mae-Ja Park Jin-Kwan Han Taejoon Kwon Tae-Joo Park Hyun-Shik Lee |
| author_facet | Soomin Chae Hyun-Kyung Lee Yoo-Kyung Kim Hyo Jung Sim Yoorim Ji Chowon Kim Tayaba Ismail Jeen-Woo Park Oh-Shin Kwon Beom-Sik Kang Dong-Seok Lee Jong-Sup Bae Sang-Hyun Kim Kyoung-Jin Min Taeg Kyu Kwon Mae-Ja Park Jin-Kwan Han Taejoon Kwon Tae-Joo Park Hyun-Shik Lee |
| author_sort | Soomin Chae |
| collection | DOAJ |
| description | Abstract Peroxiredoxin1 (Prdx1) is an antioxidant enzyme belonging to the peroxiredoxin family of proteins. Prdx1 catalyzes the reduction of H2O2 and alkyl hydroperoxide and plays an important role in different biological processes. Prdx1 also participates in various age-related diseases and cancers. In this study, we investigated the role of Prdx1 in pronephros development during embryogenesis. Prdx1 knockdown markedly inhibited proximal tubule formation in the pronephros and significantly increased the cellular levels of reactive oxygen species (ROS), which impaired primary cilia formation. Additionally, treatment with ROS (H2O2) severely disrupted proximal tubule formation, whereas Prdx1 overexpression reversed the ROS-mediated inhibition in proximal tubule formation. Epistatic analysis revealed that Prdx1 has a crucial role in retinoic acid and Wnt signaling pathways during pronephrogenesis. In conclusion, Prdx1 facilitates proximal tubule formation during pronephrogenesis by regulating ROS levels. |
| first_indexed | 2024-12-19T04:41:53Z |
| format | Article |
| id | doaj.art-e477afed45f94379b672ac594535e46d |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T04:41:53Z |
| publishDate | 2017-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-e477afed45f94379b672ac594535e46d2022-12-21T20:35:36ZengNature PortfolioScientific Reports2045-23222017-08-017111010.1038/s41598-017-09262-6Peroxiredoxin1, a novel regulator of pronephros development, influences retinoic acid and Wnt signaling by controlling ROS levelsSoomin Chae0Hyun-Kyung Lee1Yoo-Kyung Kim2Hyo Jung Sim3Yoorim Ji4Chowon Kim5Tayaba Ismail6Jeen-Woo Park7Oh-Shin Kwon8Beom-Sik Kang9Dong-Seok Lee10Jong-Sup Bae11Sang-Hyun Kim12Kyoung-Jin Min13Taeg Kyu Kwon14Mae-Ja Park15Jin-Kwan Han16Taejoon Kwon17Tae-Joo Park18Hyun-Shik Lee19KNU-Center for Nonlinear Dynamics, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National UniversityKNU-Center for Nonlinear Dynamics, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National UniversityKNU-Center for Nonlinear Dynamics, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National UniversitySchool of Life Sciences, Ulsan National Institute of Science and Technology (UNIST)KNU-Center for Nonlinear Dynamics, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National UniversityKNU-Center for Nonlinear Dynamics, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National UniversityKNU-Center for Nonlinear Dynamics, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National UniversityKNU-Center for Nonlinear Dynamics, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National UniversityKNU-Center for Nonlinear Dynamics, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National UniversityKNU-Center for Nonlinear Dynamics, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National UniversityKNU-Center for Nonlinear Dynamics, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National UniversityCollege of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National UniversityDepartment of Pharmacology, College of Medicine, Kyungpook National UniversityDepartment of Immunology, School of Medicine, Keimyung UniversityDepartment of Immunology, School of Medicine, Keimyung UniversityDepartment of Anatomy, College of Medicine, Kyungpook National UniversityDepartment of Life Sciences, Pohang University of Science and TechnologySchool of Life Sciences, Ulsan National Institute of Science and Technology (UNIST)School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST)KNU-Center for Nonlinear Dynamics, School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National UniversityAbstract Peroxiredoxin1 (Prdx1) is an antioxidant enzyme belonging to the peroxiredoxin family of proteins. Prdx1 catalyzes the reduction of H2O2 and alkyl hydroperoxide and plays an important role in different biological processes. Prdx1 also participates in various age-related diseases and cancers. In this study, we investigated the role of Prdx1 in pronephros development during embryogenesis. Prdx1 knockdown markedly inhibited proximal tubule formation in the pronephros and significantly increased the cellular levels of reactive oxygen species (ROS), which impaired primary cilia formation. Additionally, treatment with ROS (H2O2) severely disrupted proximal tubule formation, whereas Prdx1 overexpression reversed the ROS-mediated inhibition in proximal tubule formation. Epistatic analysis revealed that Prdx1 has a crucial role in retinoic acid and Wnt signaling pathways during pronephrogenesis. In conclusion, Prdx1 facilitates proximal tubule formation during pronephrogenesis by regulating ROS levels.https://doi.org/10.1038/s41598-017-09262-6 |
| spellingShingle | Soomin Chae Hyun-Kyung Lee Yoo-Kyung Kim Hyo Jung Sim Yoorim Ji Chowon Kim Tayaba Ismail Jeen-Woo Park Oh-Shin Kwon Beom-Sik Kang Dong-Seok Lee Jong-Sup Bae Sang-Hyun Kim Kyoung-Jin Min Taeg Kyu Kwon Mae-Ja Park Jin-Kwan Han Taejoon Kwon Tae-Joo Park Hyun-Shik Lee Peroxiredoxin1, a novel regulator of pronephros development, influences retinoic acid and Wnt signaling by controlling ROS levels Scientific Reports |
| title | Peroxiredoxin1, a novel regulator of pronephros development, influences retinoic acid and Wnt signaling by controlling ROS levels |
| title_full | Peroxiredoxin1, a novel regulator of pronephros development, influences retinoic acid and Wnt signaling by controlling ROS levels |
| title_fullStr | Peroxiredoxin1, a novel regulator of pronephros development, influences retinoic acid and Wnt signaling by controlling ROS levels |
| title_full_unstemmed | Peroxiredoxin1, a novel regulator of pronephros development, influences retinoic acid and Wnt signaling by controlling ROS levels |
| title_short | Peroxiredoxin1, a novel regulator of pronephros development, influences retinoic acid and Wnt signaling by controlling ROS levels |
| title_sort | peroxiredoxin1 a novel regulator of pronephros development influences retinoic acid and wnt signaling by controlling ros levels |
| url | https://doi.org/10.1038/s41598-017-09262-6 |
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