3D molecular phenotyping of cleared human brain tissues with light-sheet fluorescence microscopy

3D molecular phenotyping of cleared human brain tissues with light-sheet fluorescence microscopy

An optimized clearing approach, named SHORT, applied to thick sections of human cortical tissue allows reconstruction of large portions of the tissues using light-sheet fluorescence microscopy, by performing multicolor immunostaining and multirounds.

Bibliographic Details
Main Authors: Luca Pesce, Marina Scardigli, Vladislav Gavryusev, Annunziatina Laurino, Giacomo Mazzamuto, Niamh Brady, Giuseppe Sancataldo, Ludovico Silvestri, Christophe Destrieux, Patrick R. Hof, Irene Costantini, Francesco S. Pavone
Format: Article
Language:English
Published: Nature Portfolio 2022-05-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-022-03390-0

Internet

https://doi.org/10.1038/s42003-022-03390-0

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