Costunolide prevents renal ischemia-reperfusion injury in rats by reducing autophagy, apoptosis, inflammation, and DNA damage
Objective(s): Renal ischemia-reperfusion (I/R) is a vital health condition leading to acute kidney injury. Costunolide (COST) is an actively used molecule clinically for its anti-inflammatory, antioxidant, and immunomodulatory properties. In the present study, we searched for the possible protective...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2023-10-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | https://ijbms.mums.ac.ir/article_22554_c058e9719e6da96bb59e41e98db2be07.pdf |
| _version_ | 1797682701200785408 |
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| author | Mustafa Güler Erol Akpinar Ayhan Tanyeli Selim Çomakli Yasin Bayir |
| author_facet | Mustafa Güler Erol Akpinar Ayhan Tanyeli Selim Çomakli Yasin Bayir |
| author_sort | Mustafa Güler |
| collection | DOAJ |
| description | Objective(s): Renal ischemia-reperfusion (I/R) is a vital health condition leading to acute kidney injury. Costunolide (COST) is an actively used molecule clinically for its anti-inflammatory, antioxidant, and immunomodulatory properties. In the present study, we searched for the possible protective effects of COST against renal ischemia/reperfusion (I/R) injury in rats.Materials and Methods: We established a renal I/R rat model. We divided forty rats into four groups: group I (sham), group II (I/R), group III (I/R+COST 5 mg/kg), and group IV (I/R+COST 10 mg/kg). We collected blood, kidney, and lung samples for analysis. Results: COST administration performed anti-oxidant and anti-inflammatory activity by reducing oxidant parameters and proinflammatory cytokine levels. COST alleviated DNA damage through declining 8-hydroxydeoxyguanosine (8-OHdG) levels. In addition, COST diminished tubular damage and inflammation by reducing kidney injury molecule-1 (KIM-1) production. COST administration also ameliorated apoptosis and autophagy by decreasing caspase-3 and microtubule-associated protein light chain 3B (MAPLC3, LC3B) expression.Conclusion: COST demonstrated protective effects against renal I/R-induced injury. |
| first_indexed | 2024-03-12T00:03:38Z |
| format | Article |
| id | doaj.art-e4a8085f1a01486c9ec99a0fa45b1c1a |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-03-12T00:03:38Z |
| publishDate | 2023-10-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-e4a8085f1a01486c9ec99a0fa45b1c1a2023-09-17T06:59:57ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742023-10-0126101168117610.22038/ijbms.2023.71779.1559622554Costunolide prevents renal ischemia-reperfusion injury in rats by reducing autophagy, apoptosis, inflammation, and DNA damageMustafa Güler0Erol Akpinar1Ayhan Tanyeli2Selim Çomakli3Yasin Bayir4Department of Physiology, Faculty of Medicine, Atatürk University, Erzurum, TurkeyDepartment of Pharmacology, Faculty of Medicine, Atatürk University, Erzurum, TurkeyDepartment of Physiology, Faculty of Medicine, Atatürk University, Erzurum, TurkeyDepartment of Pathology, Faculty of Veterinary, Atatürk University, Erzurum, TurkeyDepartment of Biochemistry, Faculty of Pharmacy, Atatürk University, Erzurum, TurkeyObjective(s): Renal ischemia-reperfusion (I/R) is a vital health condition leading to acute kidney injury. Costunolide (COST) is an actively used molecule clinically for its anti-inflammatory, antioxidant, and immunomodulatory properties. In the present study, we searched for the possible protective effects of COST against renal ischemia/reperfusion (I/R) injury in rats.Materials and Methods: We established a renal I/R rat model. We divided forty rats into four groups: group I (sham), group II (I/R), group III (I/R+COST 5 mg/kg), and group IV (I/R+COST 10 mg/kg). We collected blood, kidney, and lung samples for analysis. Results: COST administration performed anti-oxidant and anti-inflammatory activity by reducing oxidant parameters and proinflammatory cytokine levels. COST alleviated DNA damage through declining 8-hydroxydeoxyguanosine (8-OHdG) levels. In addition, COST diminished tubular damage and inflammation by reducing kidney injury molecule-1 (KIM-1) production. COST administration also ameliorated apoptosis and autophagy by decreasing caspase-3 and microtubule-associated protein light chain 3B (MAPLC3, LC3B) expression.Conclusion: COST demonstrated protective effects against renal I/R-induced injury.https://ijbms.mums.ac.ir/article_22554_c058e9719e6da96bb59e41e98db2be07.pdfapoptosisautophagycostunolideratrenal ischemia-reperfusion |
| spellingShingle | Mustafa Güler Erol Akpinar Ayhan Tanyeli Selim Çomakli Yasin Bayir Costunolide prevents renal ischemia-reperfusion injury in rats by reducing autophagy, apoptosis, inflammation, and DNA damage Iranian Journal of Basic Medical Sciences apoptosis autophagy costunolide rat renal ischemia-reperfusion |
| title | Costunolide prevents renal ischemia-reperfusion injury in rats by reducing autophagy, apoptosis, inflammation, and DNA damage |
| title_full | Costunolide prevents renal ischemia-reperfusion injury in rats by reducing autophagy, apoptosis, inflammation, and DNA damage |
| title_fullStr | Costunolide prevents renal ischemia-reperfusion injury in rats by reducing autophagy, apoptosis, inflammation, and DNA damage |
| title_full_unstemmed | Costunolide prevents renal ischemia-reperfusion injury in rats by reducing autophagy, apoptosis, inflammation, and DNA damage |
| title_short | Costunolide prevents renal ischemia-reperfusion injury in rats by reducing autophagy, apoptosis, inflammation, and DNA damage |
| title_sort | costunolide prevents renal ischemia reperfusion injury in rats by reducing autophagy apoptosis inflammation and dna damage |
| topic | apoptosis autophagy costunolide rat renal ischemia-reperfusion |
| url | https://ijbms.mums.ac.ir/article_22554_c058e9719e6da96bb59e41e98db2be07.pdf |
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