Stage-Specific Non-Coding RNA Expression Patterns during In Vitro Human B Cell Differentiation into Antibody Secreting Plasma Cells
The differentiation of B cells into antibody secreting plasma cells (PCs) is governed by a strict regulatory network that results in expression of specific transcriptomes along the activation continuum. In vitro models yielding significant numbers of PCs phenotypically identical to the in vivo state...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Non-Coding RNA |
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| Online Access: | https://www.mdpi.com/2311-553X/8/1/15 |
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| author | Renee C. Tschumper Dominique B. Hoelzinger Denise K. Walters Jaime I. Davila Collin A. Osborne Diane F. Jelinek |
| author_facet | Renee C. Tschumper Dominique B. Hoelzinger Denise K. Walters Jaime I. Davila Collin A. Osborne Diane F. Jelinek |
| author_sort | Renee C. Tschumper |
| collection | DOAJ |
| description | The differentiation of B cells into antibody secreting plasma cells (PCs) is governed by a strict regulatory network that results in expression of specific transcriptomes along the activation continuum. In vitro models yielding significant numbers of PCs phenotypically identical to the in vivo state enable investigation of pathways, metabolomes, and non-coding (ncRNAs) not previously identified. The objective of our study was to characterize ncRNA expression during human B cell activation and differentiation. To achieve this, we used an in vitro system and performed RNA-seq on resting and activated B cells and PCs. Characterization of coding gene transcripts, including immunoglobulin (Ig), validated our system and also demonstrated that memory B cells preferentially differentiated into PCs. Importantly, we identified more than 980 ncRNA transcripts that are differentially expressed across the stages of activation and differentiation, some of which are known to target transcription, proliferation, cytoskeletal, autophagy and proteasome pathways. Interestingly, ncRNAs located within Ig loci may be targeting both Ig and non-Ig-related transcripts. ncRNAs associated with B cell malignancies were also identified. Taken together, this system provides a platform to study the role of specific ncRNAs in B cell differentiation and altered expression of those ncRNAs involved in B cell malignancies. |
| first_indexed | 2024-03-09T21:19:19Z |
| format | Article |
| id | doaj.art-e4b126b878d84e90930c0886e96b0646 |
| institution | Directory Open Access Journal |
| issn | 2311-553X |
| language | English |
| last_indexed | 2024-03-09T21:19:19Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Non-Coding RNA |
| spelling | doaj.art-e4b126b878d84e90930c0886e96b06462023-11-23T21:27:19ZengMDPI AGNon-Coding RNA2311-553X2022-02-01811510.3390/ncrna8010015Stage-Specific Non-Coding RNA Expression Patterns during In Vitro Human B Cell Differentiation into Antibody Secreting Plasma CellsRenee C. Tschumper0Dominique B. Hoelzinger1Denise K. Walters2Jaime I. Davila3Collin A. Osborne4Diane F. Jelinek5Department of Immunology, Mayo Clinic, Rochester, MN 55905, USADepartment of Immunology, Mayo Clinic, 13400 East Shea Boulevard, Scottsdale, AZ 85259, USADepartment of Immunology, Mayo Clinic, Rochester, MN 55905, USADepartment of Quantitative Health Sciences, Mayo Clinic, Rochester, MN 55905, USADepartment of Quantitative Health Sciences, Mayo Clinic, Rochester, MN 55905, USADepartment of Immunology, Mayo Clinic, 13400 East Shea Boulevard, Scottsdale, AZ 85259, USAThe differentiation of B cells into antibody secreting plasma cells (PCs) is governed by a strict regulatory network that results in expression of specific transcriptomes along the activation continuum. In vitro models yielding significant numbers of PCs phenotypically identical to the in vivo state enable investigation of pathways, metabolomes, and non-coding (ncRNAs) not previously identified. The objective of our study was to characterize ncRNA expression during human B cell activation and differentiation. To achieve this, we used an in vitro system and performed RNA-seq on resting and activated B cells and PCs. Characterization of coding gene transcripts, including immunoglobulin (Ig), validated our system and also demonstrated that memory B cells preferentially differentiated into PCs. Importantly, we identified more than 980 ncRNA transcripts that are differentially expressed across the stages of activation and differentiation, some of which are known to target transcription, proliferation, cytoskeletal, autophagy and proteasome pathways. Interestingly, ncRNAs located within Ig loci may be targeting both Ig and non-Ig-related transcripts. ncRNAs associated with B cell malignancies were also identified. Taken together, this system provides a platform to study the role of specific ncRNAs in B cell differentiation and altered expression of those ncRNAs involved in B cell malignancies.https://www.mdpi.com/2311-553X/8/1/15non-coding RNAplasma cellsin vitro B cell differentiationhuman B lymphocytesRNA-seq |
| spellingShingle | Renee C. Tschumper Dominique B. Hoelzinger Denise K. Walters Jaime I. Davila Collin A. Osborne Diane F. Jelinek Stage-Specific Non-Coding RNA Expression Patterns during In Vitro Human B Cell Differentiation into Antibody Secreting Plasma Cells Non-Coding RNA non-coding RNA plasma cells in vitro B cell differentiation human B lymphocytes RNA-seq |
| title | Stage-Specific Non-Coding RNA Expression Patterns during In Vitro Human B Cell Differentiation into Antibody Secreting Plasma Cells |
| title_full | Stage-Specific Non-Coding RNA Expression Patterns during In Vitro Human B Cell Differentiation into Antibody Secreting Plasma Cells |
| title_fullStr | Stage-Specific Non-Coding RNA Expression Patterns during In Vitro Human B Cell Differentiation into Antibody Secreting Plasma Cells |
| title_full_unstemmed | Stage-Specific Non-Coding RNA Expression Patterns during In Vitro Human B Cell Differentiation into Antibody Secreting Plasma Cells |
| title_short | Stage-Specific Non-Coding RNA Expression Patterns during In Vitro Human B Cell Differentiation into Antibody Secreting Plasma Cells |
| title_sort | stage specific non coding rna expression patterns during in vitro human b cell differentiation into antibody secreting plasma cells |
| topic | non-coding RNA plasma cells in vitro B cell differentiation human B lymphocytes RNA-seq |
| url | https://www.mdpi.com/2311-553X/8/1/15 |
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