Catalpol ameliorates CFA-induced inflammatory pain by targeting spinal cord and peripheral inflammation
Chronic, inflammatory pain is an international health concern that severely diminishes individuals’ quality of life. Catalpol is an iridoid glycoside derived from the roots of Rehmannia glutinosa that possesses anti-inflammatory, antioxidant, and neuroprotective properties for the treating multiple...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2022.1010483/full |
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| author | Baoxia Zhao Baoxia Zhao Jie Fu Jie Fu Huadong Ni Longsheng Xu Chengfei Xu Qiuli He Chaobo Ni Yahui Wang Jiao Kuang Mengjie Tang Qiyang Shou Ming Yao Ming Yao |
| author_facet | Baoxia Zhao Baoxia Zhao Jie Fu Jie Fu Huadong Ni Longsheng Xu Chengfei Xu Qiuli He Chaobo Ni Yahui Wang Jiao Kuang Mengjie Tang Qiyang Shou Ming Yao Ming Yao |
| author_sort | Baoxia Zhao |
| collection | DOAJ |
| description | Chronic, inflammatory pain is an international health concern that severely diminishes individuals’ quality of life. Catalpol is an iridoid glycoside derived from the roots of Rehmannia glutinosa that possesses anti-inflammatory, antioxidant, and neuroprotective properties for the treating multiple kinds of disorders. Nevertheless, catalpol’s impacts on inflammatory pain and its potential methods of action are still unclear. The purpose of this investigation is to determine the mechanism of catalpol to reduce the inflammatory pain behaviors in a rat model with complete Freund’s adjuvant (CFA). Catwalk, Von-Frey, and open field testing were performed for behavioral assessment. Western blot analysis and real-time quantitative PCR (RT-PCR) were employed to identify variations in molecular expression, while immunofluorescence was utilized to identify cellular localization. Catalpol effectively reduced CFA-induced mechanical allodynia and thermal hyperalgesia when injected intrathecally. Moreover, catalpol can regulate the HDAC4/PPAR-γ-signaling pathway in CFA rat spinal cord neurons. Meanwhile catalpol significantly decreased the expression of the NF-κB/NLRP3 inflammatory axis in the spinal cord of CFA rats. In addition, both in vivo and in vitro research revealed that catalpol treatment inhibited astrocyte activation and increase inflammatory factor expression. Interestingly, we also found that catalpol could alleviate peripheral pain by inhibiting tissue inflammation. Taken together, the findings declared that catalpol may inhibit inflammatory pain in CFA rats by targeting spinal cord and peripheral inflammation. |
| first_indexed | 2024-04-12T11:53:11Z |
| format | Article |
| id | doaj.art-e4b6e5723d32464d909587af167e5395 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-12T11:53:11Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-e4b6e5723d32464d909587af167e53952022-12-22T03:34:06ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122022-10-011310.3389/fphar.2022.10104831010483Catalpol ameliorates CFA-induced inflammatory pain by targeting spinal cord and peripheral inflammationBaoxia Zhao0Baoxia Zhao1Jie Fu2Jie Fu3Huadong Ni4Longsheng Xu5Chengfei Xu6Qiuli He7Chaobo Ni8Yahui Wang9Jiao Kuang10Mengjie Tang11Qiyang Shou12Ming Yao13Ming Yao14School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, China.Department of Anesthesiology and Pain Research Center, The First Hospital of Jiaxing Or The Affiliated Hospital of Jiaxing University, Jiaxing, ChinaSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, China.Department of Anesthesiology and Pain Research Center, The First Hospital of Jiaxing Or The Affiliated Hospital of Jiaxing University, Jiaxing, ChinaDepartment of Anesthesiology and Pain Research Center, The First Hospital of Jiaxing Or The Affiliated Hospital of Jiaxing University, Jiaxing, ChinaDepartment of Anesthesiology and Pain Research Center, The First Hospital of Jiaxing Or The Affiliated Hospital of Jiaxing University, Jiaxing, ChinaDepartment of Anesthesiology and Pain Research Center, The First Hospital of Jiaxing Or The Affiliated Hospital of Jiaxing University, Jiaxing, ChinaDepartment of Anesthesiology and Pain Research Center, The First Hospital of Jiaxing Or The Affiliated Hospital of Jiaxing University, Jiaxing, ChinaDepartment of Anesthesiology and Pain Research Center, The First Hospital of Jiaxing Or The Affiliated Hospital of Jiaxing University, Jiaxing, ChinaDepartment of Anesthesiology and Pain Research Center, The First Hospital of Jiaxing Or The Affiliated Hospital of Jiaxing University, Jiaxing, ChinaDepartment of Anesthesiology and Pain Research Center, The First Hospital of Jiaxing Or The Affiliated Hospital of Jiaxing University, Jiaxing, ChinaDepartment of Anesthesiology and Pain Research Center, The First Hospital of Jiaxing Or The Affiliated Hospital of Jiaxing University, Jiaxing, ChinaSchool of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, China.School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, China.Department of Anesthesiology and Pain Research Center, The First Hospital of Jiaxing Or The Affiliated Hospital of Jiaxing University, Jiaxing, ChinaChronic, inflammatory pain is an international health concern that severely diminishes individuals’ quality of life. Catalpol is an iridoid glycoside derived from the roots of Rehmannia glutinosa that possesses anti-inflammatory, antioxidant, and neuroprotective properties for the treating multiple kinds of disorders. Nevertheless, catalpol’s impacts on inflammatory pain and its potential methods of action are still unclear. The purpose of this investigation is to determine the mechanism of catalpol to reduce the inflammatory pain behaviors in a rat model with complete Freund’s adjuvant (CFA). Catwalk, Von-Frey, and open field testing were performed for behavioral assessment. Western blot analysis and real-time quantitative PCR (RT-PCR) were employed to identify variations in molecular expression, while immunofluorescence was utilized to identify cellular localization. Catalpol effectively reduced CFA-induced mechanical allodynia and thermal hyperalgesia when injected intrathecally. Moreover, catalpol can regulate the HDAC4/PPAR-γ-signaling pathway in CFA rat spinal cord neurons. Meanwhile catalpol significantly decreased the expression of the NF-κB/NLRP3 inflammatory axis in the spinal cord of CFA rats. In addition, both in vivo and in vitro research revealed that catalpol treatment inhibited astrocyte activation and increase inflammatory factor expression. Interestingly, we also found that catalpol could alleviate peripheral pain by inhibiting tissue inflammation. Taken together, the findings declared that catalpol may inhibit inflammatory pain in CFA rats by targeting spinal cord and peripheral inflammation.https://www.frontiersin.org/articles/10.3389/fphar.2022.1010483/fullcatalpolinflammatory painHDAC4/PPAR-γ -signaling pathwayNF-κB/NLRP3 inflammatory axisastrocyte activationperipheral pain |
| spellingShingle | Baoxia Zhao Baoxia Zhao Jie Fu Jie Fu Huadong Ni Longsheng Xu Chengfei Xu Qiuli He Chaobo Ni Yahui Wang Jiao Kuang Mengjie Tang Qiyang Shou Ming Yao Ming Yao Catalpol ameliorates CFA-induced inflammatory pain by targeting spinal cord and peripheral inflammation Frontiers in Pharmacology catalpol inflammatory pain HDAC4/PPAR-γ -signaling pathway NF-κB/NLRP3 inflammatory axis astrocyte activation peripheral pain |
| title | Catalpol ameliorates CFA-induced inflammatory pain by targeting spinal cord and peripheral inflammation |
| title_full | Catalpol ameliorates CFA-induced inflammatory pain by targeting spinal cord and peripheral inflammation |
| title_fullStr | Catalpol ameliorates CFA-induced inflammatory pain by targeting spinal cord and peripheral inflammation |
| title_full_unstemmed | Catalpol ameliorates CFA-induced inflammatory pain by targeting spinal cord and peripheral inflammation |
| title_short | Catalpol ameliorates CFA-induced inflammatory pain by targeting spinal cord and peripheral inflammation |
| title_sort | catalpol ameliorates cfa induced inflammatory pain by targeting spinal cord and peripheral inflammation |
| topic | catalpol inflammatory pain HDAC4/PPAR-γ -signaling pathway NF-κB/NLRP3 inflammatory axis astrocyte activation peripheral pain |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2022.1010483/full |
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