Near full-length HIV sequencing in multiple tissues collected postmortem reveals shared clonal expansions across distinct reservoirs during ART
Summary: HIV persists in tissues during antiretroviral therapy (ART), but the relative contribution of different anatomical compartments to the viral reservoir in humans remains unknown. We performed an extensive characterization of HIV reservoirs in two men who donated their bodies to HIV cure rese...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124723010641 |
| _version_ | 1827823102617190400 |
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| author | Caroline Dufour Maria Julia Ruiz Amélie Pagliuzza Corentin Richard Aniqa Shahid Rémi Fromentin Rosalie Ponte Amélie Cattin Tomas Raul Wiche Salinas Syim Salahuddin Teslin Sandstrom Stephanie Burke Schinkel Cecilia T. Costiniuk Mohammad-Ali Jenabian Petronela Ancuta Jean-Pierre Routy Éric A. Cohen Zabrina L. Brumme Christopher Power Jonathan B. Angel Nicolas Chomont |
| author_facet | Caroline Dufour Maria Julia Ruiz Amélie Pagliuzza Corentin Richard Aniqa Shahid Rémi Fromentin Rosalie Ponte Amélie Cattin Tomas Raul Wiche Salinas Syim Salahuddin Teslin Sandstrom Stephanie Burke Schinkel Cecilia T. Costiniuk Mohammad-Ali Jenabian Petronela Ancuta Jean-Pierre Routy Éric A. Cohen Zabrina L. Brumme Christopher Power Jonathan B. Angel Nicolas Chomont |
| author_sort | Caroline Dufour |
| collection | DOAJ |
| description | Summary: HIV persists in tissues during antiretroviral therapy (ART), but the relative contribution of different anatomical compartments to the viral reservoir in humans remains unknown. We performed an extensive characterization of HIV reservoirs in two men who donated their bodies to HIV cure research and who had been on suppressive ART for years. HIV DNA is detected in all tissues, with large variations across anatomical compartments and between participants. Intact HIV genomes represent 2% and 25% of all proviruses in the two participants and are mainly detected in secondary lymphoid organs, with the spleen and mediastinal lymph nodes harboring intact viral genomes in both individuals. Multiple copies of identical HIV genomes are found in all tissues, indicating that clonal expansions are common in anatomical sites. The majority (>85%) of these expanded clones are shared across multiple tissues. These findings suggest that infected cells expand, migrate, and possibly circulate between anatomical sites. |
| first_indexed | 2024-03-12T02:06:39Z |
| format | Article |
| id | doaj.art-e4e8b6c77a184552ad1a6aff83a1287f |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-03-12T02:06:39Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-e4e8b6c77a184552ad1a6aff83a1287f2023-09-07T04:43:48ZengElsevierCell Reports2211-12472023-09-01429113053Near full-length HIV sequencing in multiple tissues collected postmortem reveals shared clonal expansions across distinct reservoirs during ARTCaroline Dufour0Maria Julia Ruiz1Amélie Pagliuzza2Corentin Richard3Aniqa Shahid4Rémi Fromentin5Rosalie Ponte6Amélie Cattin7Tomas Raul Wiche Salinas8Syim Salahuddin9Teslin Sandstrom10Stephanie Burke Schinkel11Cecilia T. Costiniuk12Mohammad-Ali Jenabian13Petronela Ancuta14Jean-Pierre Routy15Éric A. Cohen16Zabrina L. Brumme17Christopher Power18Jonathan B. Angel19Nicolas Chomont20Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC, Canada; Centre de Recherche du CHUM, Montreal, QC CanadaDépartement de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC, Canada; Centre de Recherche du CHUM, Montreal, QC CanadaCentre de Recherche du CHUM, Montreal, QC CanadaCentre de Recherche du CHUM, Montreal, QC CanadaFaculty of Health Sciences, Simon Fraser University, Burnaby, BC, Canada; British Columbia Centre for Excellence in HIV/AIDS, Vancouver, BC, CanadaDépartement de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC, Canada; Centre de Recherche du CHUM, Montreal, QC CanadaChronic Viral Illness Service, McGill University Health Centre, Montreal, QC, Canada; Research Institute of McGill University Health Centre, Montreal, QC, CanadaDépartement de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC, Canada; Centre de Recherche du CHUM, Montreal, QC CanadaDépartement de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC, Canada; Centre de Recherche du CHUM, Montreal, QC CanadaDépartement de Sciences Biologiques, Université du Québec à Montréal, Montreal, QC, CanadaOttawa Hospital Research Institute, Ottawa, ON, Canada; Department of Biochemistry, Microbiology & Immunology, University of Ottawa, Ottawa, ON, CanadaDepartment of Biochemistry, Microbiology & Immunology, University of Ottawa, Ottawa, ON, CanadaChronic Viral Illness Service, McGill University Health Centre, Montreal, QC, Canada; Research Institute of McGill University Health Centre, Montreal, QC, CanadaDépartement de Sciences Biologiques, Université du Québec à Montréal, Montreal, QC, CanadaDépartement de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC, Canada; Centre de Recherche du CHUM, Montreal, QC CanadaResearch Institute of McGill University Health Centre, Montreal, QC, Canada; Division of Hematology, McGill University Health Centre, Montreal, QC, CanadaDépartement de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC, Canada; Institut de Recherches Cliniques de Montréal, Montreal, QC, CanadaFaculty of Health Sciences, Simon Fraser University, Burnaby, BC, Canada; British Columbia Centre for Excellence in HIV/AIDS, Vancouver, BC, CanadaDepartment of Medicine (Neurology), University of Alberta, 6-11 Heritage Medical Research Center, Edmonton, AB, Canada; Department of Psychiatry, University of Alberta, Edmonton, AB, CanadaOttawa Hospital Research Institute, Ottawa, ON, Canada; Department of Biochemistry, Microbiology & Immunology, University of Ottawa, Ottawa, ON, Canada; Division of Infectious Diseases, Ottawa Hospital-General Campus, Ottawa, ON, CanadaDépartement de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC, Canada; Centre de Recherche du CHUM, Montreal, QC Canada; Corresponding authorSummary: HIV persists in tissues during antiretroviral therapy (ART), but the relative contribution of different anatomical compartments to the viral reservoir in humans remains unknown. We performed an extensive characterization of HIV reservoirs in two men who donated their bodies to HIV cure research and who had been on suppressive ART for years. HIV DNA is detected in all tissues, with large variations across anatomical compartments and between participants. Intact HIV genomes represent 2% and 25% of all proviruses in the two participants and are mainly detected in secondary lymphoid organs, with the spleen and mediastinal lymph nodes harboring intact viral genomes in both individuals. Multiple copies of identical HIV genomes are found in all tissues, indicating that clonal expansions are common in anatomical sites. The majority (>85%) of these expanded clones are shared across multiple tissues. These findings suggest that infected cells expand, migrate, and possibly circulate between anatomical sites.http://www.sciencedirect.com/science/article/pii/S2211124723010641CP: Microbiology |
| spellingShingle | Caroline Dufour Maria Julia Ruiz Amélie Pagliuzza Corentin Richard Aniqa Shahid Rémi Fromentin Rosalie Ponte Amélie Cattin Tomas Raul Wiche Salinas Syim Salahuddin Teslin Sandstrom Stephanie Burke Schinkel Cecilia T. Costiniuk Mohammad-Ali Jenabian Petronela Ancuta Jean-Pierre Routy Éric A. Cohen Zabrina L. Brumme Christopher Power Jonathan B. Angel Nicolas Chomont Near full-length HIV sequencing in multiple tissues collected postmortem reveals shared clonal expansions across distinct reservoirs during ART Cell Reports CP: Microbiology |
| title | Near full-length HIV sequencing in multiple tissues collected postmortem reveals shared clonal expansions across distinct reservoirs during ART |
| title_full | Near full-length HIV sequencing in multiple tissues collected postmortem reveals shared clonal expansions across distinct reservoirs during ART |
| title_fullStr | Near full-length HIV sequencing in multiple tissues collected postmortem reveals shared clonal expansions across distinct reservoirs during ART |
| title_full_unstemmed | Near full-length HIV sequencing in multiple tissues collected postmortem reveals shared clonal expansions across distinct reservoirs during ART |
| title_short | Near full-length HIV sequencing in multiple tissues collected postmortem reveals shared clonal expansions across distinct reservoirs during ART |
| title_sort | near full length hiv sequencing in multiple tissues collected postmortem reveals shared clonal expansions across distinct reservoirs during art |
| topic | CP: Microbiology |
| url | http://www.sciencedirect.com/science/article/pii/S2211124723010641 |
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