Agrobacterium- and a single Cas9-sgRNA transcript system-mediated high efficiency gene editing in perennial ryegrass

Agrobacterium- and a single Cas9-sgRNA transcript system-mediated high efficiency gene editing in perennial ryegrass

Genome editing technologies provide a powerful tool for genetic improvement of perennial ryegrass, an important forage and turfgrass species worldwide. The sole publication for gene editing in perennial ryegrass used gene-gun for plant transformation and a dual promoter based CRISPR/Cas9 system for...

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Bibliographic Details
Main Authors: Rahul Kumar, Troy Kamuda, Roshani Budhathoki, Dan Tang, Huseyin Yer, Yunde Zhao, Yi Li
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-09-01
Series:Frontiers in Genome Editing
Subjects:
perennial ryegrass
single promoter
CRISPR/Cas9
PDS
ZmUbi1
ruby
Online Access:https://www.frontiersin.org/articles/10.3389/fgeed.2022.960414/full

Internet

https://www.frontiersin.org/articles/10.3389/fgeed.2022.960414/full

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