Using human iPSC-derived kidney organoids to decipher SARS-CoV-2 pathology on single cell level

Using human iPSC-derived kidney organoids to decipher SARS-CoV-2 pathology on single cell level

Summary: We describe a protocol for single-cell RNA sequencing of SARS-CoV-2-infected human induced pluripotent stem cell (iPSC)-derived kidney organoids. After inoculation of kidney organoids with virus, we use mechanical and enzymatic disruption to obtain single cell suspensions. Next, we process...

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Bibliographic Details
Main Authors: Katharina C. Reimer, Jitske Jansen, Gijs J. Overheul, Pascal Miesen, Ronald P. van Rij, Sergio H. Triana, Bart Smeets, Rebekka K. Schneider, Rafael Kramann
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
Cell biology
Single cell
Microbiology
Stem cells
Organoids
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722004920

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http://www.sciencedirect.com/science/article/pii/S2666166722004920

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