Measuring the acidification of the phagosomal lumen in live C. elegans embryos

Summary: In metazoans, the acidification of the phagosomal lumen is essential for the efficient degradation of cargoes. Here, we present a protocol for measuring the rate of acidification inside phagosomal lumen containing apoptotic cells in living C. elegans embryos. We describe steps for generatin...

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Bibliographic Details
Main Authors: Omar Peña-Ramos, Zheng Zhou
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672300299X