A Quantitative Imaging-Based Protocol for Yeast Growth and Survival on Agar Plates

Summary: We present a detailed protocol that describes the evaluation of the growth and survival of yeast cells by quantitatively analyzing spotting assays. This simple method reproducibly detects and quantifies subtle differences in growth by measuring the density of cells within a single spot of d...

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Bibliographic Details
Main Authors: Andrey A. Petropavlovskiy, Michael G. Tauro, Patrick Lajoie, Martin L. Duennwald
Format: Article
Language:English
Published: Elsevier 2020-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720301696