Evaluation of an alternative technique for preserving crustaceans in dry conditions with joint mobility: a proposal for didactic purposes
Although crustaceans are traditionally preserved in liquids (formaldehyde and/or ethyl alcohol), those substances tend to alter their morphological aspects. Glycerin, used in human anatomy, is considered a good substitute for formaldehyde, as it preserves animals in states similar to in vivo condit...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Maringá
2021-04-01
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| Series: | Acta Scientiarum: Biological Sciences |
| Subjects: | |
| Online Access: | https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/53450 |
| _version_ | 1818233667564077056 |
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| author | Amanda Brito Costa Márcio Borba da Silva Ricardo Evangelista Fraga Agda Alves da Rocha Patrícia Belini Nishiyama Matheus Santos dos Anjos Joaquim Jorge Silveira Buchaim Mariane Amorim Rocha |
| author_facet | Amanda Brito Costa Márcio Borba da Silva Ricardo Evangelista Fraga Agda Alves da Rocha Patrícia Belini Nishiyama Matheus Santos dos Anjos Joaquim Jorge Silveira Buchaim Mariane Amorim Rocha |
| author_sort | Amanda Brito Costa |
| collection | DOAJ |
| description |
Although crustaceans are traditionally preserved in liquids (formaldehyde and/or ethyl alcohol), those substances tend to alter their morphological aspects. Glycerin, used in human anatomy, is considered a good substitute for formaldehyde, as it preserves animals in states similar to in vivo conditions. There are no records in the literature, however, concerning the use of glycerin for conserving invertebrates. The objective of this work was to elaborate and evaluate alternative techniques for conserving the crustacean Ucides cordatus (Linnaeus, 1763). Six fixatives (1, 3, 4 and 5% formaldehyde, 70% alcohol, and dietrich solution) and two controls (positive and negative) were tested, as well as the effects of freezing before fixation on the integrity of U. cordatus specimens. Our results were evaluated with respect to nine variables. The treatments that demonstrated the best aesthetic results were 4% formaldehyde and 70% ethyl alcohol. The freezing of the animals resulted in brittle organs in all treatments tested. The technique discussed here is extremely promising for the conservation of animals for educational purposes, as it produces preserved specimens that are aesthetically similar to their in vivo conditions.
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| first_indexed | 2024-12-12T11:25:49Z |
| format | Article |
| id | doaj.art-e54a2a4a514e4de1a8a1d4eeaaa29a7b |
| institution | Directory Open Access Journal |
| issn | 1679-9283 1807-863X |
| language | English |
| last_indexed | 2024-12-12T11:25:49Z |
| publishDate | 2021-04-01 |
| publisher | Universidade Estadual de Maringá |
| record_format | Article |
| series | Acta Scientiarum: Biological Sciences |
| spelling | doaj.art-e54a2a4a514e4de1a8a1d4eeaaa29a7b2022-12-22T00:25:55ZengUniversidade Estadual de MaringáActa Scientiarum: Biological Sciences1679-92831807-863X2021-04-0143110.4025/actascibiolsci.v43i1.5345053450Evaluation of an alternative technique for preserving crustaceans in dry conditions with joint mobility: a proposal for didactic purposes Amanda Brito Costa0Márcio Borba da Silva1Ricardo Evangelista Fraga2Agda Alves da Rocha3Patrícia Belini Nishiyama4Matheus Santos dos Anjos5Joaquim Jorge Silveira Buchaim6Mariane Amorim Rocha7Universidade Federal da BahiaUniversidade Federal da Bahia Universidade Federal da BahiaUniversidade Federal da Bahia Universidade Federal da BahiaUniversidade Federal da Bahia Faculdade Anglo-AmericanoUniversidade Federal da Bahia Although crustaceans are traditionally preserved in liquids (formaldehyde and/or ethyl alcohol), those substances tend to alter their morphological aspects. Glycerin, used in human anatomy, is considered a good substitute for formaldehyde, as it preserves animals in states similar to in vivo conditions. There are no records in the literature, however, concerning the use of glycerin for conserving invertebrates. The objective of this work was to elaborate and evaluate alternative techniques for conserving the crustacean Ucides cordatus (Linnaeus, 1763). Six fixatives (1, 3, 4 and 5% formaldehyde, 70% alcohol, and dietrich solution) and two controls (positive and negative) were tested, as well as the effects of freezing before fixation on the integrity of U. cordatus specimens. Our results were evaluated with respect to nine variables. The treatments that demonstrated the best aesthetic results were 4% formaldehyde and 70% ethyl alcohol. The freezing of the animals resulted in brittle organs in all treatments tested. The technique discussed here is extremely promising for the conservation of animals for educational purposes, as it produces preserved specimens that are aesthetically similar to their in vivo conditions. https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/53450preservation; glycerin; Ucides cordatus; teaching. |
| spellingShingle | Amanda Brito Costa Márcio Borba da Silva Ricardo Evangelista Fraga Agda Alves da Rocha Patrícia Belini Nishiyama Matheus Santos dos Anjos Joaquim Jorge Silveira Buchaim Mariane Amorim Rocha Evaluation of an alternative technique for preserving crustaceans in dry conditions with joint mobility: a proposal for didactic purposes Acta Scientiarum: Biological Sciences preservation; glycerin; Ucides cordatus; teaching. |
| title | Evaluation of an alternative technique for preserving crustaceans in dry conditions with joint mobility: a proposal for didactic purposes |
| title_full | Evaluation of an alternative technique for preserving crustaceans in dry conditions with joint mobility: a proposal for didactic purposes |
| title_fullStr | Evaluation of an alternative technique for preserving crustaceans in dry conditions with joint mobility: a proposal for didactic purposes |
| title_full_unstemmed | Evaluation of an alternative technique for preserving crustaceans in dry conditions with joint mobility: a proposal for didactic purposes |
| title_short | Evaluation of an alternative technique for preserving crustaceans in dry conditions with joint mobility: a proposal for didactic purposes |
| title_sort | evaluation of an alternative technique for preserving crustaceans in dry conditions with joint mobility a proposal for didactic purposes |
| topic | preservation; glycerin; Ucides cordatus; teaching. |
| url | https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/53450 |
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