The Anti-Photoaging Activity of Peptides from <i>Pinctada martensii</i> Meat
Long-term exposure to ultraviolet-B (UVB) can cause photoaging. Peptides from <i>Pinctada martensii</i> meat have been shown to have anti-photoaging activities, but their mechanism of action is rarely studied. In this study, <i>Pinctada martensii</i> meat hydrolysates (PME) w...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-12-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1660-3397/20/12/770 |
| _version_ | 1797456622661926912 |
|---|---|
| author | Mengfen Wei Huamai Qiu Jie Zhou Chenghao Yang Yifan Chen Lijun You |
| author_facet | Mengfen Wei Huamai Qiu Jie Zhou Chenghao Yang Yifan Chen Lijun You |
| author_sort | Mengfen Wei |
| collection | DOAJ |
| description | Long-term exposure to ultraviolet-B (UVB) can cause photoaging. Peptides from <i>Pinctada martensii</i> meat have been shown to have anti-photoaging activities, but their mechanism of action is rarely studied. In this study, <i>Pinctada martensii</i> meat hydrolysates (PME) were prepared by digestive enzymes and then separated by ultrafiltration and Sephadex G-25 gel filtration chromatography to obtain a purified fraction (G2). The fraction G2 was identified by ultra-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS), and peptide sequences were synthesized by solid-phase synthesis. The mechanism of anti-photoaging activities was investigated using a human immortalised epidermal (HaCaT) cell model. Results showed that peptides from <i>Pinctada martensii</i> meat increased UVB-induced cell viability and reduced the contents of interstitial collagenase (MMP-1) and matrix lysing enzyme (MMP-3) in HaCaT cells. Furthermore, the fraction of G2 significantly downregulated the expression of <i>p38</i>, <i>EKR</i>, <i>JNK</i>, <i>MMP-1</i>, and <i>MMP-3</i> in HaCaT cells. The peptide sequences Phe-His (FH), Ala-Leu (AL), Met-Tyr (MY), Ala-Gly-Phe (AGF), and Ile-Tyr-Pro (IYP) were identified and synthesized. Besides, FH reduced the contents of MMP-1 and MMP-3 in HaCaT cells, combining them effectively in molecular docking analysis. Thus, peptides from <i>Pinctada martensii</i> meat showed anti-photoaging activities and might have the potential to be used as an anti-photoaging agent in functional foods. |
| first_indexed | 2024-03-09T16:10:29Z |
| format | Article |
| id | doaj.art-e55ab9b678ab48f59f6652312454459f |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-09T16:10:29Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-e55ab9b678ab48f59f6652312454459f2023-11-24T16:19:54ZengMDPI AGMarine Drugs1660-33972022-12-01201277010.3390/md20120770The Anti-Photoaging Activity of Peptides from <i>Pinctada martensii</i> MeatMengfen Wei0Huamai Qiu1Jie Zhou2Chenghao Yang3Yifan Chen4Lijun You5School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, ChinaSchool of Food Science and Engineering, South China University of Technology, Guangzhou 510640, ChinaSchool of Food Science and Engineering, South China University of Technology, Guangzhou 510640, ChinaIra A. Fulton Schools of Engineering, Arizona State University, Tempe, AZ 85281, USASchool of Food Science and Engineering, South China University of Technology, Guangzhou 510640, ChinaSchool of Food Science and Engineering, South China University of Technology, Guangzhou 510640, ChinaLong-term exposure to ultraviolet-B (UVB) can cause photoaging. Peptides from <i>Pinctada martensii</i> meat have been shown to have anti-photoaging activities, but their mechanism of action is rarely studied. In this study, <i>Pinctada martensii</i> meat hydrolysates (PME) were prepared by digestive enzymes and then separated by ultrafiltration and Sephadex G-25 gel filtration chromatography to obtain a purified fraction (G2). The fraction G2 was identified by ultra-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS), and peptide sequences were synthesized by solid-phase synthesis. The mechanism of anti-photoaging activities was investigated using a human immortalised epidermal (HaCaT) cell model. Results showed that peptides from <i>Pinctada martensii</i> meat increased UVB-induced cell viability and reduced the contents of interstitial collagenase (MMP-1) and matrix lysing enzyme (MMP-3) in HaCaT cells. Furthermore, the fraction of G2 significantly downregulated the expression of <i>p38</i>, <i>EKR</i>, <i>JNK</i>, <i>MMP-1</i>, and <i>MMP-3</i> in HaCaT cells. The peptide sequences Phe-His (FH), Ala-Leu (AL), Met-Tyr (MY), Ala-Gly-Phe (AGF), and Ile-Tyr-Pro (IYP) were identified and synthesized. Besides, FH reduced the contents of MMP-1 and MMP-3 in HaCaT cells, combining them effectively in molecular docking analysis. Thus, peptides from <i>Pinctada martensii</i> meat showed anti-photoaging activities and might have the potential to be used as an anti-photoaging agent in functional foods.https://www.mdpi.com/1660-3397/20/12/770<i>Pinctada martensii</i>peptidephotoagingHaCaTmolecular docking |
| spellingShingle | Mengfen Wei Huamai Qiu Jie Zhou Chenghao Yang Yifan Chen Lijun You The Anti-Photoaging Activity of Peptides from <i>Pinctada martensii</i> Meat Marine Drugs <i>Pinctada martensii</i> peptide photoaging HaCaT molecular docking |
| title | The Anti-Photoaging Activity of Peptides from <i>Pinctada martensii</i> Meat |
| title_full | The Anti-Photoaging Activity of Peptides from <i>Pinctada martensii</i> Meat |
| title_fullStr | The Anti-Photoaging Activity of Peptides from <i>Pinctada martensii</i> Meat |
| title_full_unstemmed | The Anti-Photoaging Activity of Peptides from <i>Pinctada martensii</i> Meat |
| title_short | The Anti-Photoaging Activity of Peptides from <i>Pinctada martensii</i> Meat |
| title_sort | anti photoaging activity of peptides from i pinctada martensii i meat |
| topic | <i>Pinctada martensii</i> peptide photoaging HaCaT molecular docking |
| url | https://www.mdpi.com/1660-3397/20/12/770 |
| work_keys_str_mv | AT mengfenwei theantiphotoagingactivityofpeptidesfromipinctadamartensiiimeat AT huamaiqiu theantiphotoagingactivityofpeptidesfromipinctadamartensiiimeat AT jiezhou theantiphotoagingactivityofpeptidesfromipinctadamartensiiimeat AT chenghaoyang theantiphotoagingactivityofpeptidesfromipinctadamartensiiimeat AT yifanchen theantiphotoagingactivityofpeptidesfromipinctadamartensiiimeat AT lijunyou theantiphotoagingactivityofpeptidesfromipinctadamartensiiimeat AT mengfenwei antiphotoagingactivityofpeptidesfromipinctadamartensiiimeat AT huamaiqiu antiphotoagingactivityofpeptidesfromipinctadamartensiiimeat AT jiezhou antiphotoagingactivityofpeptidesfromipinctadamartensiiimeat AT chenghaoyang antiphotoagingactivityofpeptidesfromipinctadamartensiiimeat AT yifanchen antiphotoagingactivityofpeptidesfromipinctadamartensiiimeat AT lijunyou antiphotoagingactivityofpeptidesfromipinctadamartensiiimeat |