Improved efficacy of SARS-CoV-2 isolation from COVID-19 clinical specimens using VeroE6 cells overexpressing TMPRSS2 and human ACE2

Improved efficacy of SARS-CoV-2 isolation from COVID-19 clinical specimens using VeroE6 cells overexpressing TMPRSS2 and human ACE2

Abstract The cell culture-based isolation of novel coronavirus SARS-CoV-2 from clinical specimens obtained from patients with suspected COVID-19 is important not only for laboratory diagnosis but also for obtaining live virus to characterize emerging variants. Previous studies report that monkey kid...

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Bibliographic Details
Main Authors: Hitomi Kinoshita, Tsukasa Yamamoto, Yudai Kuroda, Yusuke Inoue, Kaya Miyazaki, Norio Ohmagari, Daisuke Tokita, Phu Hoang Anh Nguyen, Souichi Yamada, Shizuko Harada, Takayuki Kanno, Kenichiro Takahashi, Masumichi Saito, Kazuya Shirato, Ikuyo Takayama, Shinji Watanabe, Tomoya Saito, Hideki Ebihara, Tadaki Suzuki, Ken Maeda, Shuetsu Fukushi
Format: Article
Language:English
Published: Nature Portfolio 2024-10-01
Series:Scientific Reports
Subjects:
SARS-CoV-2
Virus isolation
ACE2
TMPRSS2
Online Access:https://doi.org/10.1038/s41598-024-75038-4

Internet

https://doi.org/10.1038/s41598-024-75038-4

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